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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-8-3
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pubmed:abstractText |
A ribozyme gene directed at a specific cleavage of mRNA coding for PB1 protein, a component of RNA-dependent RNA-polymerase of influenza A virus, was constructed. The avian adenovirus CELO virus-associated RNA (VA RNA CELO) promoter and human cytomegalovirus (CMV) promoter were used for the permanent expression of the ribozyme in cell lines. The cells were infected with influenza A virus strains A/Singapore/1/57 and A/WSN/33, and the suppression of the virus reproduction and virus-specific protein synthesis was measured. The maximal level of the inhibition of virus reproduction as compared to the reproduction in non-transformed cells was 93.5%. Defective recombinant adenoviruses were constructed carrying the genes of functional and non-functional ribozymes under the control of human cytomegalovirus (CMV) promoter. The reproduction of A/WSN/33 virus in CV-1 cells preinfected with recombinant adenoviruses was shown to be suppressed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0166-3542
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
47-57
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10333142-Adenoviridae,
pubmed-meshheading:10333142-Animals,
pubmed-meshheading:10333142-Base Sequence,
pubmed-meshheading:10333142-Cell Line,
pubmed-meshheading:10333142-Cytomegalovirus,
pubmed-meshheading:10333142-Humans,
pubmed-meshheading:10333142-Influenza A virus,
pubmed-meshheading:10333142-Molecular Sequence Data,
pubmed-meshheading:10333142-Plasmids,
pubmed-meshheading:10333142-Promoter Regions, Genetic,
pubmed-meshheading:10333142-RNA, Catalytic,
pubmed-meshheading:10333142-RNA, Messenger,
pubmed-meshheading:10333142-RNA, Viral,
pubmed-meshheading:10333142-RNA Replicase,
pubmed-meshheading:10333142-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10333142-Transfection,
pubmed-meshheading:10333142-Viral Proteins,
pubmed-meshheading:10333142-Virus Replication
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pubmed:year |
1999
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pubmed:articleTitle |
Inhibition of influenza A virus reproduction by a ribozyme targeted against PB1 mRNA.
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pubmed:affiliation |
Institute of Agricultural Biotechnology, Russian Academy of Agricultural Science, Moscow.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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