Linked Life Data

10331945

Source:http://linkedlifedata.com/resource/pubmed/id/10331945

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-7-6
pubmed:databankReference
pubmed:abstractText
We report the sequence of the guinea pig p53 cDNA. The comparative analysis of the coding and noncoding regions of p53 cDNAs of all available complete vertebrate sequences has allowed us to single out new conserved signals possibly involved in p53 functional activity. We have focused our attention on the most variable region of the protein, the proline (P)-rich domain, suggested to play a fundamental role in antiproliferative pathways. In this domain we have identified the PXXXXP repeated motif and singled out a common consensus sequence that can be considered a signature for mammalian p53: PXXXXPX{0,4}PX{0,9}PA(T,P,I,)(S,P)WPL. We have demonstrated the significance of the PXXXXP motif in SH3-binding protein and suggested its structure to be a loop. Also, the 5' and 3' untranslated regions (UTRs) of the guinea pig were sequenced, and this study represents the first detailed structural analysis of the UTRs of the p53 mRNAs available in literature. The 5' UTR of guinea pig (233 nt) can be folded into a stable secondary structure resembling that predicted in mouse. The 3' UTR of guinea pig is 771 nt long and shows higher similarity with human than with rodent sequences, having a region of about 350 nt that is deleted in rat and mouse. In the 3' UTR we have identified the presence of a mammalian-wide interspersed repeat sequence and of a cytoplasmic polyadenylation element, which could be involved in translational activation by promoting polyadenylation of mRNA, providing information about a possible mechanism of regulation of p53 expression mediated by the 3' UTR of the mRNA. The observations presented here could open new avenues to targeted mutations and experimental approaches useful in investigating new regulation mechanisms of p53 translation, activity, and stability.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0888-7543
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
50-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10331945-3' Untranslated Regions, pubmed-meshheading:10331945-5' Untranslated Regions, pubmed-meshheading:10331945-Amino Acid Sequence, pubmed-meshheading:10331945-Animals, pubmed-meshheading:10331945-Base Sequence, pubmed-meshheading:10331945-DNA, Complementary, pubmed-meshheading:10331945-Evolution, Molecular, pubmed-meshheading:10331945-Guinea Pigs, pubmed-meshheading:10331945-Models, Molecular, pubmed-meshheading:10331945-Molecular Sequence Data, pubmed-meshheading:10331945-Nucleic Acid Conformation, pubmed-meshheading:10331945-RNA, Messenger, pubmed-meshheading:10331945-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:10331945-Sequence Alignment, pubmed-meshheading:10331945-Sequence Analysis, DNA, pubmed-meshheading:10331945-Sequence Homology, Amino Acid, pubmed-meshheading:10331945-Sequence Homology, Nucleic Acid, pubmed-meshheading:10331945-Tumor Suppressor Protein p53
pubmed:year
1999
pubmed:articleTitle
Guinea pig p53 mRNA: identification of new elements in coding and untranslated regions and their functional and evolutionary implications.
pubmed:affiliation
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Via Orabona 4, Bari, 70126, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't