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pubmed-article:10329475pubmed:abstractTextUnsaturated fatty acids are essential lipid components of Mucor rouxii. Gamma-linolenic acid (GLA) is synthesized via the desaturase enzymes: delta9-desaturase catalyzes mono-unsaturated fatty acids that are utilized as substrate for GLA biosynthesis. We cloned and characterized a M. rouxii gene highly homologous to delta9-desaturase genes. This sequence encodes for a protein of 452 amino acids and contains two introns of 60 and 61 nucleotides. Delta9-desaturase of M. rouxii is expressed during cell growth when cells are subjected to temperature shifts. At 30 degrees C, the mRNA level of late log phase is about 6.4-fold higher than that of early log phase. A shift from 30 to 15 degrees C induced transcription of delta9-desaturase gene in both early and late log phases. However, the pattern of increased transcription by cold induction varied depending on growth conditions: transcription of late log phase is higher than that of early log phase. These results indicate that cell growth and low temperature influence the expression of delta9-desaturase gene and fatty acid composition of M. rouxii.lld:pubmed
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pubmed-article:10329475pubmed:dateRevised2009-11-19lld:pubmed
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pubmed-article:10329475pubmed:articleTitleMucor rouxii delta9-desaturase gene is transcriptionally regulated during cell growth and by low temperature.lld:pubmed
pubmed-article:10329475pubmed:affiliationSchool of Bioresources and Technology, King Mongkut's University of Technology, Thonburi, Bangkok, Thailand.lld:pubmed
pubmed-article:10329475pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10329475pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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