Linked Life Data

10329475

Source:http://linkedlifedata.com/resource/pubmed/id/10329475

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-9-21
pubmed:abstractText
Unsaturated fatty acids are essential lipid components of Mucor rouxii. Gamma-linolenic acid (GLA) is synthesized via the desaturase enzymes: delta9-desaturase catalyzes mono-unsaturated fatty acids that are utilized as substrate for GLA biosynthesis. We cloned and characterized a M. rouxii gene highly homologous to delta9-desaturase genes. This sequence encodes for a protein of 452 amino acids and contains two introns of 60 and 61 nucleotides. Delta9-desaturase of M. rouxii is expressed during cell growth when cells are subjected to temperature shifts. At 30 degrees C, the mRNA level of late log phase is about 6.4-fold higher than that of early log phase. A shift from 30 to 15 degrees C induced transcription of delta9-desaturase gene in both early and late log phases. However, the pattern of increased transcription by cold induction varied depending on growth conditions: transcription of late log phase is higher than that of early log phase. These results indicate that cell growth and low temperature influence the expression of delta9-desaturase gene and fatty acid composition of M. rouxii.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1522-4724
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
36-43
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10329475-Amino Acid Sequence, pubmed-meshheading:10329475-Base Sequence, pubmed-meshheading:10329475-Cell Division, pubmed-meshheading:10329475-Conserved Sequence, pubmed-meshheading:10329475-DNA Primers, pubmed-meshheading:10329475-Fatty Acids, pubmed-meshheading:10329475-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10329475-Gene Expression Regulation, Fungal, pubmed-meshheading:10329475-Genes, Fungal, pubmed-meshheading:10329475-Lipid Metabolism, pubmed-meshheading:10329475-Molecular Sequence Data, pubmed-meshheading:10329475-Mucor, pubmed-meshheading:10329475-RNA, Fungal, pubmed-meshheading:10329475-RNA, Messenger, pubmed-meshheading:10329475-Sequence Homology, Amino Acid, pubmed-meshheading:10329475-Stearoyl-CoA Desaturase, pubmed-meshheading:10329475-Temperature
pubmed:year
1999
pubmed:articleTitle
Mucor rouxii delta9-desaturase gene is transcriptionally regulated during cell growth and by low temperature.
pubmed:affiliation
School of Bioresources and Technology, King Mongkut's University of Technology, Thonburi, Bangkok, Thailand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't