Linked Life Data

10329198

Source:http://linkedlifedata.com/resource/pubmed/id/10329198

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-6-21
pubmed:abstractText
We recently reported that the apolipoprotein B (apo-B) gene is expressed in the heart and that the heart secretes apo-B-containing lipoproteins. We have also recently demonstrated that the tissue-specific expression of the apo-B gene is complex, with apo-B gene expression in the intestine being dependent on an enhancer element located very far from the structural gene (between 54 and 62 kb 5' to exon 1). In this study, we investigated whether these distant DNA sequences control or affect the expression of the apo-B gene in the heart. To address this issue, we analysed heart apo-B gene expression in heart tissue from a series of human apo-B transgenic mice generated from human genomic DNA constructs that ranged in size from 70 to 207 kb. All of these constructs contained the coding portion of the apo-B gene, but each contained different amounts of flanking sequences (5-120 kb 5' to the gene and 1.5-35 kb 3' to the gene). Each construct conferred human apo-B gene expression in the heart, including those containing as little as 5 kb of 5'-flanking sequences or as little as 1.5 kb of 3'-flanking sequences. These data support the idea that apo-B gene expression in the heart, unlike that in the intestine, is not affected by a distant enhancer element.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2828
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
695-703
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
The DNA sequences required for apolipoprotein B expression in the heart are distinct from those required for expression in the intestine.
pubmed:affiliation
The Gladstone Institute of Cardiovascular Disease, Department of Medicine, University of California, San Francisco, CA 94141-9100, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't