|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1999-6-9
|
|
pubmed:abstractText |
We have studied the global structure of the U1A 3' untranslated region (UTR) element using fluorescence resonance energy transfer. Comparison of a single UTR-box with a series of oligoadenine bulges indicates that the UTR-box introduces a significant kink into the axis of the RNA, and quantification of the results suggests an included bend angle of approximately 100 degrees (i.e. 80 degrees from linear). The complete 3'-UTR element is also severely kinked by the two UTR-boxes. We can observe binding of U1A protein to the 3'-UTR element by a change in the fluorescence anisotropy of Cy3 attached to one of the helical ends. In parallel with the binding, we observe a marked increase in fluoresence resonance energy transfer efficiency between fluorophores attached at the two 5' termini, indicating a significant change in global conformation induced by the binding of the protein.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0022-2836
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
14
|
|
pubmed:volume |
288
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
585-94
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:10329165-3' Untranslated Regions,
pubmed-meshheading:10329165-Base Sequence,
pubmed-meshheading:10329165-DNA Primers,
pubmed-meshheading:10329165-Energy Transfer,
pubmed-meshheading:10329165-Fluorescence Polarization,
pubmed-meshheading:10329165-Nucleic Acid Conformation,
pubmed-meshheading:10329165-Protein Binding,
pubmed-meshheading:10329165-RNA, Messenger,
pubmed-meshheading:10329165-RNA Precursors,
pubmed-meshheading:10329165-RNA Splicing,
pubmed-meshheading:10329165-RNA-Binding Proteins,
pubmed-meshheading:10329165-Ribonucleoprotein, U1 Small Nuclear
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Binding of U1A protein to the 3' untranslated region of its pre-mRNA.
|
|
pubmed:affiliation |
Department of Biochemistry, The University of Dundee, Dundee, DD1 4HN, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
