Source:http://linkedlifedata.com/resource/pubmed/id/10328848
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1999-6-28
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| pubmed:abstractText |
To trace cell lineages and the origin and fate of cells in transplantation and embryo chimeras, a DNA/DNA in situ hybridization cell labelling system was developed, based on a 50-copy murine c-myc transgene on mouse chromosome 8. Elevated levels of cMyc mRNA were found in Myc*tg50 (Myctg50/0 and Myctg50/Myctg50) transgenic tissues, but adult transgenic NMRI mice were anatomically and histologically indistinguishable from control NMRI mice and did not develop tumours on a wild-type or nude (nu/nu) background. The hybridization label detected transgenic nuclei with an efficiency of approximately 80%. In muscle grafts, this transgene label was successfully applied to trace donor cells in a labelled host and to study the invasion of a graft by host cells. When the cMyc hybridization was used in allophenic mice of the control<-->NMRI-Myctg50/? (nu/+ or +/+) type, an up to a three-fold excess of MYC*tg50 positive over control nuclei was found in all organs examined (ventricle, skeletal muscle, liver, small intestine). This overgrowth of MYC*tg50 cells is probably due to transgene expression. Four out of seven (C57BL/6xBALB/c) or (C57BL/6xNMRI)<-->MYC*tg50 allophenic mice displayed anatomical abnormalities, e.g. an enlarged thymus and a tumour in the groin region. As these abnormalities were only observed in allophenic mice, they might be due to the imbalance of growth potential between MYC*tg50 transgenic and normal cells in the same individual.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1065-6995
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1998 Academic Press.
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| pubmed:issnType |
Print
|
| pubmed:volume |
22
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
401-11
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10328848-Animals,
pubmed-meshheading:10328848-Cell Nucleus,
pubmed-meshheading:10328848-Chimera,
pubmed-meshheading:10328848-Chromosome Mapping,
pubmed-meshheading:10328848-Crosses, Genetic,
pubmed-meshheading:10328848-Female,
pubmed-meshheading:10328848-Genes, myc,
pubmed-meshheading:10328848-In Situ Hybridization,
pubmed-meshheading:10328848-Intestine, Small,
pubmed-meshheading:10328848-Liver,
pubmed-meshheading:10328848-Male,
pubmed-meshheading:10328848-Mice,
pubmed-meshheading:10328848-Mice, Inbred BALB C,
pubmed-meshheading:10328848-Mice, Inbred C57BL,
pubmed-meshheading:10328848-Mice, Inbred Strains,
pubmed-meshheading:10328848-Mice, Transgenic,
pubmed-meshheading:10328848-Muscle, Skeletal,
pubmed-meshheading:10328848-Myocardium,
pubmed-meshheading:10328848-Neoplasms, Experimental,
pubmed-meshheading:10328848-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:10328848-RNA, Messenger,
pubmed-meshheading:10328848-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10328848-Transcription, Genetic
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| pubmed:year |
1998
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| pubmed:articleTitle |
A multicopy c-Myc transgene as a nuclear label: overgrowth of Myctg50 cells in allophenic mice.
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| pubmed:affiliation |
Developmental Biology and Molecular Pathology, University of Bielefeld, Bielefeld, D-33501, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|