Linked Life Data

10326676

Source:http://linkedlifedata.com/resource/pubmed/id/10326676

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-7-23
pubmed:abstractText
We investigated the expression and the subcellular localization of S100A1 and S100B, two Ca(2+)-binding proteins of the EF-hand type, in replicating myoblasts and fused myotubes. Northern blot and reverse transcriptase-polymerase chain reaction analyses revealed the presence of S100A1 mRNA and S100B mRNA respectively, in myoblasts. Immunofluorescence and immunogold electron microscopy were used to localize individual proteins in myoblasts and myotubes. In the present report we document that: (1) in replicating myoblasts S100B is localized to intracellular membranes, including Golgi membranes, vimentin intermediate filaments (IFs) and microtubule (MT) structures; (2) in the same cells S100A1 is found associated with intracellular membranes; (3) following treatment of replicating myoblasts with colchicine, a fraction of S100B remains colocalized with bundled and collapsed vimentin IFs, whereas another fraction follows the destiny of endoplasmic membranes; (4) under the same conditions S100A1, like a fraction of S100B, follows the collapse of the endoplasmic reticulum around the nucleus; and (5) in fused myotubes S100A1 is found diffusely in the cytoplasm, whereas S100B is mostly found associated with vimentin IFs. These data suggest that in the skeletal myogenic cell line used in the present study S100A1 and S100B might share binding sites on or close to intracellular membranes, but display a significant degree of target specificity with respect of IFs and MTs. The results of these analyses suggest that expression of S100B in skeletal muscle cells may be developmentally regulated and lend support to the possibility that S100B might regulate the MT and IF dynamics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0143-4160
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-106
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10326676-Amino Acid Sequence, pubmed-meshheading:10326676-Animals, pubmed-meshheading:10326676-Antineoplastic Agents, Phytogenic, pubmed-meshheading:10326676-Base Sequence, pubmed-meshheading:10326676-Blotting, Northern, pubmed-meshheading:10326676-Calcium, pubmed-meshheading:10326676-Calcium-Binding Proteins, pubmed-meshheading:10326676-Cell Line, pubmed-meshheading:10326676-Cytoplasm, pubmed-meshheading:10326676-Fluorescent Antibody Technique, pubmed-meshheading:10326676-Golgi Apparatus, pubmed-meshheading:10326676-Intermediate Filaments, pubmed-meshheading:10326676-Intracellular Membranes, pubmed-meshheading:10326676-Microtubules, pubmed-meshheading:10326676-Molecular Sequence Data, pubmed-meshheading:10326676-Muscle, Skeletal, pubmed-meshheading:10326676-Nerve Growth Factors, pubmed-meshheading:10326676-Paclitaxel, pubmed-meshheading:10326676-Rats, pubmed-meshheading:10326676-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10326676-S100 Proteins, pubmed-meshheading:10326676-Vimentin
pubmed:year
1999
pubmed:articleTitle
Replicating myoblasts and fused myotubes express the calcium-regulated proteins S100A1 and S100B.
pubmed:affiliation
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't