Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5417
pubmed:dateCreated
1999-6-8
pubmed:abstractText
Coinjection of unfertilized mouse oocytes with sperm heads and exogenous DNA encoding either a green fluorescent protein (GFP) or beta-galactosidase reporter produced 64 to 94 percent transgene-expressing embryos, reflecting DNA-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos in the GFP series generated about 20 percent offspring expressing the integrated transgene. These data indicate that exogenous DNA can reproducibly be delivered into an oocyte by microinjected spermatozoa and suggest an adaptable method of transgenesis.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1180-3
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10325231-Animals, pubmed-meshheading:10325231-Blastocyst, pubmed-meshheading:10325231-Cell Membrane, pubmed-meshheading:10325231-Culture Techniques, pubmed-meshheading:10325231-Embryo Transfer, pubmed-meshheading:10325231-Embryonic and Fetal Development, pubmed-meshheading:10325231-Female, pubmed-meshheading:10325231-Fertilization in Vitro, pubmed-meshheading:10325231-Gene Transfer Techniques, pubmed-meshheading:10325231-Genes, Reporter, pubmed-meshheading:10325231-Green Fluorescent Proteins, pubmed-meshheading:10325231-Lac Operon, pubmed-meshheading:10325231-Luminescent Proteins, pubmed-meshheading:10325231-Male, pubmed-meshheading:10325231-Mice, pubmed-meshheading:10325231-Mice, Transgenic, pubmed-meshheading:10325231-Microinjections, pubmed-meshheading:10325231-Morula, pubmed-meshheading:10325231-Oocytes, pubmed-meshheading:10325231-Sperm Head, pubmed-meshheading:10325231-Transgenes
pubmed:year
1999
pubmed:articleTitle
Mammalian transgenesis by intracytoplasmic sperm injection.
pubmed:affiliation
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, HI 96822, USA. perry@hawaii.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't