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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-6-7
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pubmed:abstractText |
Production of angiotensin II (Ang II) in spontaneously hypertensive rats (SHR)-derived vascular smooth muscle cells (VSMC) has now been investigated. A nonpeptide antagonist (CV-11974) of Ang II type 1 receptors inhibited basal DNA synthesis in VSMC from SHR, but it had no effect on cells from Wistar-Kyoto (WKY) rats. Ang II-like immunoreactivity, determined by radioimmunoassay after HPLC, was readily detected in conditioned medium and extracts of SHR-derived VSMC, whereas it was virtually undetectable in VSMC from WKY rats. Isoproterenol increased the amount of Ang II-like immunoreactivity in conditioned medium and extracts of SHR-derived VSMC, whereas the angiotensin-converting enzyme inhibitor delapril significantly reduced the amount of Ang II-like immunoreactivity in conditioned medium and extracts of these cells. Reverse transcription-polymerase chain reaction analysis revealed that the abundance of mRNAs encoding angiotensinogen, cathepsin D, and angiotensin-converting enzyme was greater in VSMC from SHR than in cells from WKY rats. The abundance of cathepsin D protein by Western blotting was greater in VSMC from SHR than in cells from WKY rats. Ang I-generating and acid protease activities were detected in VSMC from SHR, but not in cells from WKY rats. These results suggest that SHR-derived VSMC generate Ang II with increases in angiotensinogen, cathepsin D, and angiotensin-converting enzyme, which contribute to the basal growth. Production of Ang II by homogeneous cultures of VSMC is considered as a new mechanism of hypertensive vascular disease.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin-Converting Enzyme...,
http://linkedlifedata.com/resource/pubmed/chemical/Antihypertensive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin D,
http://linkedlifedata.com/resource/pubmed/chemical/Cathepsin E,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Indans,
http://linkedlifedata.com/resource/pubmed/chemical/Kallikreins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidyl-Dipeptidase A,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Angiotensin, Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Angiotensin,
http://linkedlifedata.com/resource/pubmed/chemical/Renin,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Kallikreins,
http://linkedlifedata.com/resource/pubmed/chemical/candesartan,
http://linkedlifedata.com/resource/pubmed/chemical/delapril
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1079-5642
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1210-7
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10323771-Angiotensin II,
pubmed-meshheading:10323771-Angiotensin Receptor Antagonists,
pubmed-meshheading:10323771-Angiotensin-Converting Enzyme Inhibitors,
pubmed-meshheading:10323771-Animals,
pubmed-meshheading:10323771-Antihypertensive Agents,
pubmed-meshheading:10323771-Aspartic Acid Endopeptidases,
pubmed-meshheading:10323771-Benzimidazoles,
pubmed-meshheading:10323771-Cathepsin D,
pubmed-meshheading:10323771-Cathepsin E,
pubmed-meshheading:10323771-Cell Division,
pubmed-meshheading:10323771-Cells, Cultured,
pubmed-meshheading:10323771-Culture Media, Conditioned,
pubmed-meshheading:10323771-Hyperplasia,
pubmed-meshheading:10323771-Hypertension,
pubmed-meshheading:10323771-Hypertrophy,
pubmed-meshheading:10323771-Indans,
pubmed-meshheading:10323771-Kallikreins,
pubmed-meshheading:10323771-Male,
pubmed-meshheading:10323771-Muscle, Smooth, Vascular,
pubmed-meshheading:10323771-Peptidyl-Dipeptidase A,
pubmed-meshheading:10323771-RNA, Messenger,
pubmed-meshheading:10323771-Rats,
pubmed-meshheading:10323771-Rats, Inbred SHR,
pubmed-meshheading:10323771-Rats, Inbred WKY,
pubmed-meshheading:10323771-Receptor, Angiotensin, Type 1,
pubmed-meshheading:10323771-Receptor, Angiotensin, Type 2,
pubmed-meshheading:10323771-Receptors, Angiotensin,
pubmed-meshheading:10323771-Renin,
pubmed-meshheading:10323771-Renin-Angiotensin System,
pubmed-meshheading:10323771-Tetrazoles,
pubmed-meshheading:10323771-Tissue Kallikreins
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pubmed:year |
1999
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pubmed:articleTitle |
Production of angiotensin II by homogeneous cultures of vascular smooth muscle cells from spontaneously hypertensive rats.
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pubmed:affiliation |
Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo 173, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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