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rdf:type |
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lifeskim:mentions |
umls-concept:C0004359,
umls-concept:C0009782,
umls-concept:C0021024,
umls-concept:C0030705,
umls-concept:C0034790,
umls-concept:C0142661,
umls-concept:C0205250,
umls-concept:C0205369,
umls-concept:C0337112,
umls-concept:C0936012,
umls-concept:C1524075,
umls-concept:C2003941
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pubmed:issue |
3
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pubmed:dateCreated |
1999-6-23
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF009788,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF009790,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF009792,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF086764,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF086765,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF086766,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF086767,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF086768,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF086769,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF086770,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF087019,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U78307,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U83989,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U83990,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U84142,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U85050,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U85051,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U85052,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U85054,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U85394,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U85452
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pubmed:abstractText |
The U1-70kD autoantigen is a major target of B cell responses in patients with connective tissue diseases (CTD). T cell responses are important in the pathogenesis of CTD, however little is known about autoantigen-specific T cells in these diseases. We have recently proven that U1-70kD-reactive human T cells exist. To further characterize these autoreactive T cells, U1-70kD-reactive T cell clones have been generated from patients with CTD using either a recombinant fusion protein or synthetic peptides spanning the U1-70kD polypeptide. T cell receptors (TCR) isolated from the U1-70kD-reactive T cell clones were sequenced and the third complementarity-determining region (CDR3) compared to determine if a common motif was present. mAb blocking of antigen-induced proliferation was done to determine the HLA restriction element used in recognition of the U1-70kD autoantigen by T cells. The results presented here indicate that TCRAV CDR3 usage is highly restricted among U1-70kD autoantigen-specific human T cells clones derived from CTD patients with distinctive structural features. Furthermore, the recognition of the U1-70kD autoantigen occurs in the context of HLA-DR.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0198-8859
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
200-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10321956-Amino Acid Sequence,
pubmed-meshheading:10321956-Autoantigens,
pubmed-meshheading:10321956-Clone Cells,
pubmed-meshheading:10321956-Computer Simulation,
pubmed-meshheading:10321956-Connective Tissue Diseases,
pubmed-meshheading:10321956-Conserved Sequence,
pubmed-meshheading:10321956-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:10321956-HLA-DR Antigens,
pubmed-meshheading:10321956-Humans,
pubmed-meshheading:10321956-Immunoglobulin Variable Region,
pubmed-meshheading:10321956-Lupus Erythematosus, Systemic,
pubmed-meshheading:10321956-Mixed Connective Tissue Disease,
pubmed-meshheading:10321956-Models, Molecular,
pubmed-meshheading:10321956-Molecular Sequence Data,
pubmed-meshheading:10321956-Peptide Fragments,
pubmed-meshheading:10321956-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10321956-Ribonucleoprotein, U1 Small Nuclear,
pubmed-meshheading:10321956-Sequence Analysis, DNA,
pubmed-meshheading:10321956-T-Lymphocytes
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pubmed:year |
1999
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pubmed:articleTitle |
Analysis of T cell receptors specific for U1-70kD small nuclear ribonucleoprotein autoantigen: the alpha chain complementarity determining region three is highly conserved among connective tissue disease patients.
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pubmed:affiliation |
Department of Internal Medicine, University of Missouri, Department of Veterans Affairs, Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65212, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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