Linked Life Data

10320777

Source:http://linkedlifedata.com/resource/pubmed/id/10320777

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-6-17
pubmed:databankReference
pubmed:abstractText
Hypoxia-inducible factor (HIF)-1alpha and -2alpha are two basic helix-loop-helix/PAS domain transcriptional factors that mediate hypoxia-induced gene expression. We found that bovine arterial endothelial cells (BAEC) expressed both HIF-1alpha and -2alpha by RT-PCR and then isolated cDNAs encoding these two transcriptional factors of BAEC. The deduced amino acid sequences of both HIF-1alpha and -2alpha showed high homologies among mammalian species. Northern blot analysis indicated that the mRNAs for HIF-1alpha and -2alpha from BAEC showed a size of approx. 5.5 and 6.2 kilobases, respectively and that both mRNAs were constitutively expressed and not induced by hypoxia in BAEC.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
1445
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-43
pubmed:dateRevised
2007-4-11
pubmed:meshHeading
pubmed-meshheading:10320777-Amino Acid Sequence, pubmed-meshheading:10320777-Animals, pubmed-meshheading:10320777-Base Sequence, pubmed-meshheading:10320777-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:10320777-Cattle, pubmed-meshheading:10320777-Cells, Cultured, pubmed-meshheading:10320777-Cloning, Molecular, pubmed-meshheading:10320777-DNA, Complementary, pubmed-meshheading:10320777-DNA-Binding Proteins, pubmed-meshheading:10320777-Endothelium, Vascular, pubmed-meshheading:10320777-Gene Expression Regulation, pubmed-meshheading:10320777-Hypoxia-Inducible Factor 1, pubmed-meshheading:10320777-Hypoxia-Inducible Factor 1, alpha Subunit, pubmed-meshheading:10320777-Molecular Sequence Data, pubmed-meshheading:10320777-Nuclear Proteins, pubmed-meshheading:10320777-Sequence Alignment, pubmed-meshheading:10320777-Trans-Activators, pubmed-meshheading:10320777-Transcription Factors
pubmed:year
1999
pubmed:articleTitle
Molecular cloning of cDNAs encoding hypoxia-inducible factor (HIF)-1alpha and -2alpha of bovine arterial endothelial cells.
pubmed:affiliation
Department of Public Health, School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't