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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-6-15
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pubmed:abstractText |
The transcription factor Sox9 is capable of enhancing type II collagen gene expression and may play a crucial role in chondrogenesis. To determine whether Sox9 is an inducer of the chondrocyte phenotype, we investigated the role of Sox9 in transcription of another cartilage gene encoding the cartilage-derived retinoic acid-sensitive protein (CD-RAP). CD-RAP is specifically expressed during chondrogenesis. We show here that Sox9 protein is able to bind to a SOX consensus sequence in the CD-RAP promoter. Mutation of the SOX motif led to decreased transcription of a CD-RAP promoter construct in chondrocytes. Overexpression of SOX9 resulted in a dose-dependent increased activity of CD-RAP promoter-driven reporter gene in both chondrocytes and nonchondrogenic cells. A truncated SOX9, which contains a binding domain but no trans-activation function, inhibited CD-RAP promoter activity. Overexpression of SOX9 increased the level of endogenous CD-RAP mRNA in chondrocytes, but was unable to induce endogenous gene expression in 10T1/2 mesenchymal cells or BALB/c-3T3 fibroblasts. These results suggest that Sox9 is a general transcriptional regulator of cartilage-specific genes. However, Sox9 does not appear to be able to induce the chondrocyte phenotype in nonchondrogenic cells, implying that other factors are involved in chondrogenesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/High Mobility Group Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mia1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SOX9 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Sex-Determining Region Y Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Sox9 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0884-0431
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
757-63
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10320524-Animals,
pubmed-meshheading:10320524-Cartilage,
pubmed-meshheading:10320524-Cells, Cultured,
pubmed-meshheading:10320524-Chondrocytes,
pubmed-meshheading:10320524-DNA,
pubmed-meshheading:10320524-DNA-Binding Proteins,
pubmed-meshheading:10320524-Extracellular Matrix Proteins,
pubmed-meshheading:10320524-Gene Expression Regulation, Developmental,
pubmed-meshheading:10320524-High Mobility Group Proteins,
pubmed-meshheading:10320524-Mice,
pubmed-meshheading:10320524-Nuclear Proteins,
pubmed-meshheading:10320524-Phenotype,
pubmed-meshheading:10320524-Promoter Regions, Genetic,
pubmed-meshheading:10320524-Proteins,
pubmed-meshheading:10320524-SOX9 Transcription Factor,
pubmed-meshheading:10320524-Sex-Determining Region Y Protein,
pubmed-meshheading:10320524-Transcription Factors,
pubmed-meshheading:10320524-Transcriptional Activation,
pubmed-meshheading:10320524-Tretinoin
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pubmed:year |
1999
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pubmed:articleTitle |
Trans-activation of the mouse cartilage-derived retinoic acid-sensitive protein gene by Sox9.
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pubmed:affiliation |
Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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