Source:http://linkedlifedata.com/resource/pubmed/id/10235508
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
1999-5-13
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| pubmed:abstractText |
A proposed hemopoietic stem cell gene therapy for treatment for HIV infection would involve transduction of CD34+ hemopoietic stem cells with vectors encoding anti-HIV constructs. Peripheral blood has proved to be a useful source of these hemopoietic stem cells and this study exploits this finding. Small quantities of peripheral blood were obtained from HIV-negative patients and HIV-positive patients who were and were not receiving hemopoietic growth factors (HGFs). CD34+ cells were obtained from these samples using a simple technique and scored for frequency of colony type. This demonstrated that HIV-negative patients had the highest frequency of colony-forming units (CFUs). HIV-positive patients not treated with HGFs had a lower frequency of CFUs, but the same colony type distribution as HIV-negative patients. HIV-positive patients treated with HGFs had the lowest frequency of CFUs, but their colony type distribution demonstrated that they had responded to treatment. CD34+ cells selected in this way were also transduced with the murine retroviral MFG vector using a technique that demonstrated transduction efficiencies ranging from 2% to 16% (median, 11.5%). This study simplifies the experimental requirements for development of a hemopoietic stem cell gene therapy for HIV infection and offers the possibility that longitudinal studies could be performed on peripheral blood CD34+ cells from HIV-positive or HIV-negative patients without the need for granulocyte colony-stimulating factor mobilization.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1525-4135
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
21
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1-8
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10235508-Adult,
pubmed-meshheading:10235508-Antigens, CD34,
pubmed-meshheading:10235508-Cell Count,
pubmed-meshheading:10235508-Cell Culture Techniques,
pubmed-meshheading:10235508-Cell Separation,
pubmed-meshheading:10235508-Cell Transformation, Viral,
pubmed-meshheading:10235508-Coculture Techniques,
pubmed-meshheading:10235508-DNA, Viral,
pubmed-meshheading:10235508-Female,
pubmed-meshheading:10235508-Flow Cytometry,
pubmed-meshheading:10235508-Gene Therapy,
pubmed-meshheading:10235508-HIV Infections,
pubmed-meshheading:10235508-HIV-1,
pubmed-meshheading:10235508-Hematopoietic Cell Growth Factors,
pubmed-meshheading:10235508-Hematopoietic Stem Cells,
pubmed-meshheading:10235508-Humans,
pubmed-meshheading:10235508-Male,
pubmed-meshheading:10235508-Middle Aged,
pubmed-meshheading:10235508-Polymerase Chain Reaction,
pubmed-meshheading:10235508-Proviruses
|
| pubmed:year |
1999
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| pubmed:articleTitle |
Isolation and transduction of CD34+ cells from small quantities of peripheral blood from HIV-1-infected patients not treated with hemopoietic growth factors.
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| pubmed:affiliation |
Department of Genito-Urinary Medicine and Communicable Diseases, Jefferiss Research Trust Laboratories, Imperial College School of Medicine at St. Mary's, London, UK.
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| pubmed:publicationType |
Journal Article
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