Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6730
pubmed:dateCreated
1999-6-14
pubmed:abstractText
Gene activation in eukaryotes is regulated by complex mechanisms in which the recruitment and assembly of the transcriptional machinery is directed by gene- and cell-type-specific DNA-binding proteins. When DNA is packaged into chromatin, the regulation of gene activation requires new classes of chromatin-targeting activity. In humans, a multisubunit cofactor functions in a chromatin-selective manner to potentiate synergistic gene activation by the transcriptional activators SREBP-1a and Sp1. Here we show that this activator-recruited cofactor (ARC) interacts directly with several different activators, including SREBP-1a, VP16 and the p65 subunit of NF-kappaB, and strongly enhances transcription directed by these activators in vitro with chromatin-assembled DNA templates. The ARC complex consists of 16 or more subunits; some of these are novel gene products, whereas others are present in other multisubunit cofactors, such as CRSP, NAT and mammalian Mediator. Detailed analysis indicates that the ARC complex is probably identical to the nuclear hormone-receptor cofactor DRIP. Thus, ARC/DRIP is a large composite co-activator that belongs to a family of related cofactors and is targeted by different classes of activator to mediate transcriptional stimulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/CRSP protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Chromatin, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Herpes Simplex Virus Protein Vmw65, http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances, http://linkedlifedata.com/resource/pubmed/chemical/Med17 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Mediator Complex, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, LDL, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/SREBF1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/Sterol Regulatory Element Binding..., http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0028-0836
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
398
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
828-32
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10235267-Amino Acid Sequence, pubmed-meshheading:10235267-Animals, pubmed-meshheading:10235267-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:10235267-Chromatin, pubmed-meshheading:10235267-DNA-Binding Proteins, pubmed-meshheading:10235267-Escherichia coli, pubmed-meshheading:10235267-HeLa Cells, pubmed-meshheading:10235267-Herpes Simplex Virus Protein Vmw65, pubmed-meshheading:10235267-Humans, pubmed-meshheading:10235267-Macromolecular Substances, pubmed-meshheading:10235267-Mediator Complex, pubmed-meshheading:10235267-Molecular Sequence Data, pubmed-meshheading:10235267-Nuclear Proteins, pubmed-meshheading:10235267-Receptors, LDL, pubmed-meshheading:10235267-Recombinant Fusion Proteins, pubmed-meshheading:10235267-Sp1 Transcription Factor, pubmed-meshheading:10235267-Sterol Regulatory Element Binding Protein 1, pubmed-meshheading:10235267-Trans-Activators, pubmed-meshheading:10235267-Transcription Factors, pubmed-meshheading:10235267-Transcriptional Activation
pubmed:year
1999
pubmed:articleTitle
Composite co-activator ARC mediates chromatin-directed transcriptional activation.
pubmed:affiliation
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't