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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1999-6-7
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pubmed:abstractText |
The development of human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is closely associated with the activation of T cells which are HTLV-1 specific but may cross-react with neural antigens (Ags). Immature dendritic cells (DCs), differentiated from normal donor monocytes by using recombinant granulocyte-macrophage colony-stimulating factor and recombinant interleukin-4, were pulsed with HTLV-1 in vitro. The pulsed DCs contained HTLV-1 proviral DNA and expressed HTLV-1 Gag Ag on their surface 6 days after infection. The DCs matured by lipopolysaccharides stimulated autologous CD4(+) T cells and CD8(+) T cells in a viral dose-dependent manner. However, the proliferation level of CD4(+) T cells was five- to sixfold higher than that of CD8(+) T cells. In contrast to virus-infected DCs, DCs pulsed with heat-inactivated virions activated only CD4(+) T cells. To clarify the role of DCs in HAM/TSP development, monocytes from patients were cultured for 4 days in the presence of the cytokines. The expression of CD86 Ag on DCs was higher and that of CD1a Ag was more down-regulated than in DCs generated from normal monocytes. DCs from two of five patients expressed HTLV-1 Gag Ag. Furthermore, both CD4(+) and CD8(+) T cells from the patients were greatly stimulated by contact with autologous DCs pulsed with inactivated viral Ag as well as HTLV-1-infected DCs. These results suggest that DCs are susceptible to HTLV-1 infection and that their cognate interaction with T cells may contribute to the development of HAM/TSP.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-10225829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-1352913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-1457215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-2146511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-2894806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-7529992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-7537726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-7629501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-7664181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-7760009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-7910031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-7979225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-8006603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-8040335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-8102389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-8145033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-8355031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-8429100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-8455685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-9100633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-9201301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-9209495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-9294135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-9294136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-9333647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233916-9435256
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4575-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1999
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pubmed:articleTitle |
The role of human T-lymphotropic virus type 1 (HTLV-1)-infected dendritic cells in the development of HTLV-1-associated myelopathy/tropical spastic paraparesis.
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pubmed:affiliation |
Division of Human Retroviruses, Center for Chronic Viral Diseases, Faculty of Medicine, Kagoshima University, Kagoshima 890-8520, Japan. makino-m@cb3.so-net.ne.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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