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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1999-7-21
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pubmed:abstractText |
Major histocompatibility complex (MHC)/peptide association and stability are determined by specific amino acid interactions between peptide antigens and the MHC groove, and are regarded as a critical feature in ensuring efficient monitoring by T cells. In this investigation we examined the relationship between MHC/peptide stability and the immunostimulatory capacity of MHC/peptide complexes. For this purpose we compared synthetic peptide analogues derived from the immunodominant HLA-A11-presented IVTDFSVIK (IVT) epitope, for their capacity to reactivate IVT-specific memory cytotoxic T-lymphocyte (CTL) responses. The analogues differentiated from the wild-type epitope by single amino acid substitution at position 2. All peptides showed similar affinity for HLA-A11 molecules and were recognized by IVT-specific CTL clones, but induced HLA-A11 complexes at the cell surface with different lifespan. This model offered the possibility of comparing the capacity of an immunogenic epitope to stimulate a unique population of T-cell precursors depending on the lifespan of its presentation at the cell surface. We demonstrated that stable HLA-A11/peptide complexes efficiently stimulate IVT-specific CTL responses, while HLA-A11/peptide complexes with short lifespan do not. The precise identification of the role of amino acid residues in the formation of stable MHC/peptide complexes may be relevant for the design of wild-type-derived epitopes with high immunogenicity. These analogues may have important applications in the immunotherapy of infectious diseases and immunogenic tumours.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-1321426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-1709722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-2038058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-2198471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-2594067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-2666863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-3522223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7511684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7523572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7527444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7545295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7679748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7682013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7694806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-7818553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-8384560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-8384718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-8407969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-8617954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-8671682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-8706347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-9278330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10233722-9468286
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
411-5
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10233722-Cell Culture Techniques,
pubmed-meshheading:10233722-Cell Line,
pubmed-meshheading:10233722-Cytotoxicity, Immunologic,
pubmed-meshheading:10233722-Epitopes,
pubmed-meshheading:10233722-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:10233722-HLA-A Antigens,
pubmed-meshheading:10233722-HLA-A11 Antigen,
pubmed-meshheading:10233722-Half-Life,
pubmed-meshheading:10233722-Herpesvirus 4, Human,
pubmed-meshheading:10233722-Histocompatibility Antigens Class I,
pubmed-meshheading:10233722-Humans,
pubmed-meshheading:10233722-Immunologic Memory,
pubmed-meshheading:10233722-Peptide Fragments,
pubmed-meshheading:10233722-T-Lymphocytes, Cytotoxic
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pubmed:year |
1999
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pubmed:articleTitle |
The lifespan of major histocompatibility complex class I/peptide complexes determines the efficiency of cytotoxic T-lymphocyte responses.
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pubmed:affiliation |
Biochemistry and Molecular Biology, University of Ferrara, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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