Linked Life Data

10232830

Source:http://linkedlifedata.com/resource/pubmed/id/10232830

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7-8
pubmed:dateCreated
1999-6-23
pubmed:abstractText
Reactive oxygen species (ROS) inhibit sperm movement and have been implicated in male infertility. In this study, we determined the effects of specific ROS produced by activated leukocytes on human spermatozoa and investigated their metabolic site of action. We used chemiluminescence and electron paramagnetic resonance (EPR) to characterize the ROS generated by both blood and seminal leukocytes. We also determined the effects of these ROS on sperm energy metabolism using biochemical analyses and flow cytometry. Both blood and seminal leukocytes produced the same characteristic ROS which were determined to be hydrogen peroxide (H2O2) and superoxide radicals (O2*-). EPR using the spin trapping technique indicated that superoxide radical-dependent hydroxyl radicals (HO.) were also generated. ROS generated by PMA-stimulated blood leukocytes (2-5 x 10(6)/ml) caused inhibition of sperm movement in 2 h (p < .01). Using the hypoxanthine/ xanthine oxidase (0.5 U/ml) system to generate ROS, we determined that spermatozoa ATP levels, after ROS treatment, were reduced approximately eight-fold in 30 min (0.10 x 10(10) moles/10(6) sperm cells) compared to control (0.84 X 10(-10) moles/10(6) sperm cells) (p < .01). Sperm ATP reduction paralleled the inhibition of sperm forward progression. Neither superoxide dismutase (100 U/ml) nor dimethyl sulfoxide (100 mM) reversed these effects; however, protection was observed with catalase (4 X 10(3) U/ml). Flow cytometric analyses of sperm treated with various doses of H2O2 (0.3 mM-20.0 mM) showed a dose-dependent decrease in sperm mitochondrial membrane potential (MMP); however, at low concentrations of H2O2, sperm MMP was not significantly inhibited. Also, sperm MMP uncoupling with CCClP had no effect on either sperm ATP levels or forward progression. These results indicate that H2O2 is the toxic ROS produced by activated leukocytes causing the inhibition of both sperm movement and ATP production. O2*- and HO. do not play a significant role in these processes. Low concentrations of H2O2 causing complete inhibition of sperm movement and ATP levels inhibit sperm energy metabolism at a site independent of mitochondrial oxidative phosphorylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0891-5849
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
869-80
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10232830-Adenosine Triphosphate, pubmed-meshheading:10232830-Carbonyl Cyanide m-Chlorophenyl Hydrazone, pubmed-meshheading:10232830-Catalase, pubmed-meshheading:10232830-Dimethyl Sulfoxide, pubmed-meshheading:10232830-Electron Spin Resonance Spectroscopy, pubmed-meshheading:10232830-Energy Metabolism, pubmed-meshheading:10232830-Humans, pubmed-meshheading:10232830-Hydrogen Peroxide, pubmed-meshheading:10232830-Hypoxanthine, pubmed-meshheading:10232830-Leukocytes, pubmed-meshheading:10232830-Luminescent Measurements, pubmed-meshheading:10232830-Male, pubmed-meshheading:10232830-Reactive Oxygen Species, pubmed-meshheading:10232830-Sperm Motility, pubmed-meshheading:10232830-Spermatozoa, pubmed-meshheading:10232830-Superoxide Dismutase, pubmed-meshheading:10232830-Superoxides, pubmed-meshheading:10232830-Tetradecanoylphorbol Acetate, pubmed-meshheading:10232830-Xanthine Oxidase
pubmed:year
1999
pubmed:articleTitle
Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism.
pubmed:affiliation
Department of Urology, Tulane University Medical Center, New Orleans, LA, USA.
pubmed:publicationType
Journal Article, In Vitro