Source:http://linkedlifedata.com/resource/pubmed/id/10231572
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1999-6-28
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pubmed:databankReference | |
pubmed:abstractText |
Streptomyces coelicolor Müller contains two types of superoxide dismutase (SOD) containing Ni (encoded by sodN) or Fe (encoded by sodF). Unlike a single species of Fe-containing SOD in Müller strain, multiple forms of FeSODs were detected in S. coelicolor A3(2) strain by activity staining and Western blot analysis. Genomic Southern hybridization suggested the presence of at least two copies of the sodF-like gene in A3(2). Two different genes for FeSOD (sodF1 and sodF2) were isolated from the phage library of A3(2) genome. The nucleotide sequence of the sodF1 coding region was identical with the unique sodF gene from Müller while that of sodF2 shared 88% identity. The gene products of sodF1 and sodF2 were identified by activity staining and immunoblot analysis. Expression from the sodF1 gene was repressed by nickel as sensitively as Müller sodF, suggesting the presence of Ni-responsive regulatory site within the region shared by the two genes. Among 12 other Streptomyces species examined, only S. fradiae contained two FeSOD-like polypeptides. We postulate that the additional copy of the sodF gene (sodF2) was provided by the horizontal transfer from remotely related bacteria.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
231
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
87-93
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10231572-Amino Acid Sequence,
pubmed-meshheading:10231572-Base Sequence,
pubmed-meshheading:10231572-DNA, Bacterial,
pubmed-meshheading:10231572-Gene Duplication,
pubmed-meshheading:10231572-Molecular Sequence Data,
pubmed-meshheading:10231572-Restriction Mapping,
pubmed-meshheading:10231572-Sequence Homology, Amino Acid,
pubmed-meshheading:10231572-Streptomyces,
pubmed-meshheading:10231572-Superoxide Dismutase
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pubmed:year |
1999
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pubmed:articleTitle |
Duplicate genes for Fe-containing superoxide dismutase in Streptomyces coelicolor A3(2).
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pubmed:affiliation |
Department of Microbiology, College of Natural Sciences and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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