Source:http://linkedlifedata.com/resource/pubmed/id/10227995
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1999-5-20
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| pubmed:abstractText |
The adoptive transfer of TCR-transgenic T cells into syngeneic recipients allows characterization of individual T cells during in vivo immune responses. However, the proliferative behavior of individual T cells and its relationship to effector and memory function has been difficult to define. Here, we used a fluorescent dye to dissect and quantify T cell proliferative dynamics in vivo. We find that the average Ag-specific CD4+ T cell that undergoes division in vivo generates >20 daughter cells. TCR and CD28 signals cooperatively determine the degree of primary clonal expansion by increasing both the proportion of Ag-specific T cells that divide and the number of rounds of division the responding T cells undergo. Nonetheless, despite optimal signaling, up to one-third of Ag-specific cells fail to divide even though they show phenotypic evidence of Ag encounter. Surprisingly, however, transgenic T cells maturing on a RAG-2-/- background exhibit a responder frequency of 95-98% in vivo, suggesting that maximal proliferative potential requires either a naive phenotype or allelic exclusion at the TCRalpha locus. Finally, studies reveal division cycle-dependent expression of markers of T cell differentiation, such as CD44, CD45RB, and CD62L, and show also that expression of the cytokines IFN-gamma and IL-2 depends primarily on cell division rather than on receipt of costimulatory signals. These results provide a quantitative assessment of T cell proliferation in vivo and define the relationship between cell division and other parameters of the immune response including cytokine production, the availability of costimulation, and the capacity for memory.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
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| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
162
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5212-23
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| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:10227995-Adoptive Transfer,
pubmed-meshheading:10227995-Alleles,
pubmed-meshheading:10227995-Animals,
pubmed-meshheading:10227995-Antigens, CD28,
pubmed-meshheading:10227995-CD4 Lymphocyte Count,
pubmed-meshheading:10227995-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10227995-Cell Cycle,
pubmed-meshheading:10227995-Cell Differentiation,
pubmed-meshheading:10227995-Cell Division,
pubmed-meshheading:10227995-Clone Cells,
pubmed-meshheading:10227995-Cytokines,
pubmed-meshheading:10227995-Epitopes, T-Lymphocyte,
pubmed-meshheading:10227995-Lymph Nodes,
pubmed-meshheading:10227995-Lymphocyte Activation,
pubmed-meshheading:10227995-Mice,
pubmed-meshheading:10227995-Mice, Inbred BALB C,
pubmed-meshheading:10227995-Mice, Transgenic,
pubmed-meshheading:10227995-Ovalbumin,
pubmed-meshheading:10227995-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10227995-Signal Transduction,
pubmed-meshheading:10227995-T-Lymphocyte Subsets
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| pubmed:year |
1999
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| pubmed:articleTitle |
Dynamics and requirements of T cell clonal expansion in vivo at the single-cell level: effector function is linked to proliferative capacity.
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| pubmed:affiliation |
Department of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|