Source:http://linkedlifedata.com/resource/pubmed/id/10227982
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
1999-5-20
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| pubmed:abstractText |
Thymic shared Ag-2 (TSA-2) is a 28-kDa, glycophosphatidylinitosol-linked cell surface molecule expressed on various T cell and thymic stromal cell subsets. It is expressed on most CD3-CD4-CD8-, CD4+CD8+, and CD3highCD4-CD8+ thymocytes but is down-regulated on approximately 40% of CD3highCD4+CD8- thymocytes. Expression on peripheral TCR-alphabeta+ T cells is similar to that of CD3+ thymocytes, although a transient down-regulation occurs with cell activation. Consistent with the recent hypothesis that emigration from the thymus is an active process, recent thymic emigrants are primarily TSA-2-/low. TSA-2 expression reveals heterogeneity among subpopulations of CD3highCD4+CD8- thymocytes and TCR-gamma delta+ T cell previously regarded as homogenous. The functional importance of TSA-2 was illustrated by the severe block in T cell differentiation caused by adding purified anti-TSA-2 mAb to reconstituted fetal thymic organ culture. While each CD25/CD44-defined triple-negative subset was present, differentiation beyond the TN stage was essentially absent, and cell numbers of all subsets were significantly below those of control cultures. Cross-linking TSA-2 on thymocytes caused a significant Ca2+ influx but no increase in apoptosis, unless anti-TSA-2 was used in conjunction with suboptimal anti-CD3 mAb. Similar treatment of mature TSA-2+ T cells had no effect on cell survival or proliferation. This study reveals TSA-2 to be a functionally important molecule in T cell development and a novel indicator of heterogeneity among a variety of developing and mature T cell populations.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/thymic shared antigen-1
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
162
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5119-26
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10227982-Aging,
pubmed-meshheading:10227982-Animals,
pubmed-meshheading:10227982-Animals, Newborn,
pubmed-meshheading:10227982-Antibodies, Monoclonal,
pubmed-meshheading:10227982-Antigens, CD3,
pubmed-meshheading:10227982-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:10227982-Apoptosis,
pubmed-meshheading:10227982-Biological Markers,
pubmed-meshheading:10227982-Calcium,
pubmed-meshheading:10227982-Cell Differentiation,
pubmed-meshheading:10227982-Cell Movement,
pubmed-meshheading:10227982-Drug Synergism,
pubmed-meshheading:10227982-Fetus,
pubmed-meshheading:10227982-Lymphocyte Activation,
pubmed-meshheading:10227982-Membrane Proteins,
pubmed-meshheading:10227982-Mice,
pubmed-meshheading:10227982-Mice, Inbred CBA,
pubmed-meshheading:10227982-Organ Culture Techniques,
pubmed-meshheading:10227982-T-Lymphocyte Subsets,
pubmed-meshheading:10227982-Thymus Gland
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| pubmed:year |
1999
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| pubmed:articleTitle |
Thymic shared antigen-2: a novel cell surface marker associated with T cell differentiation and activation.
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| pubmed:affiliation |
Department of Pathology and Immunology, Monash Medical School, Prahran, Australia. Stuart.Berzins@med.monash.edu.au
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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