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pubmed-article:10226879pubmed:abstractTextPulse field gradient (PFG) diffusion NMR spectroscopy is a non-invasive method for the spectroscopic separation and identification of compounds of interest from a mixture. Because it relies on differences in translational diffusion rates to resolve NMR signals from individual components, pulse field gradient NMR is a unique method for analyzing complex mixtures and for detecting intermolecular interactions. A number of multidimensional pulse field gradient NMR experiments have been developed to alleviate the overlap of NMR signals arising from a complex mixture and facilitate component identification. The applications of pulse field gradient NMR for mixture analysis and for the direct identification of high affinity ligands are reviewed.lld:pubmed
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pubmed-article:10226879pubmed:authorpubmed-author:EydeR HRHlld:pubmed
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pubmed-article:10226879pubmed:dateRevised2005-11-16lld:pubmed
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pubmed-article:10226879pubmed:articleTitleNuclear magnetic resonance chromatography: applications of pulse field gradient diffusion NMR to mixture analysis and ligand-receptor interactions.lld:pubmed
pubmed-article:10226879pubmed:affiliationCore Technology Area/Analytics & Bio-NMR US, Novartis Institute for Biomedical Research, Novartis Pharmaceuticals Corporation, Summit, NJ 07901, USA.lld:pubmed
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