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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1999-6-10
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pubmed:databankReference |
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pubmed:abstractText |
It is widely believed that the surface M protein of group A streptococci is the predominant surface protein of these organisms containing opsonic epitopes. In the present study, we identified a new surface protein, distinct from M protein, that evokes protective antibodies. A type 18 M-negative mutant was found to be both resistant to phagocytosis in human blood and virulent in mice. The wild-type strain, but not the M-negative mutant, was opsonized by antisera against purified recombinant M18 protein or a synthetic peptide copying the NH2-terminus of M18. However, antisera raised against a crude pepsin extract of the M-negative mutant opsonized both strains, indicating the presence of a protective antigen in addition to type 18 M protein. This antiserum was used to identify and purify a 24-kDa protein fragment (Spa, streptococcal protective antigen) that evoked antibodies that opsonized the M18 parent and the M-negative mutant. The results of passive mouse protection tests confirmed the presence of protective epitopes within Spa. The deduced amino acid sequence of a 636-bp 5' fragment of the spa18 gene showed no homology with sequences in GenBank. These studies reveal the presence of a new protective antigen of certain strains of group A streptococci that may prove to be an important component of vaccines to prevent streptococcal infections.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-14461914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-1475516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-1634809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-1656437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-2406246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-2422314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-2446327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-2450950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-2578539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-2670192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-325168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-3807984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-4111795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-4132911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-4971603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-5922288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-6195292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-6207110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-7883175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-7927767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-8322487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-8332063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-8345202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-8613352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-8830235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-8873386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-8975893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225969-9987154
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1261-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10225969-Amino Acid Sequence,
pubmed-meshheading:10225969-Animals,
pubmed-meshheading:10225969-Antibodies, Bacterial,
pubmed-meshheading:10225969-Antigens, Bacterial,
pubmed-meshheading:10225969-Bacterial Vaccines,
pubmed-meshheading:10225969-Base Sequence,
pubmed-meshheading:10225969-Cloning, Molecular,
pubmed-meshheading:10225969-Cross Reactions,
pubmed-meshheading:10225969-DNA, Bacterial,
pubmed-meshheading:10225969-Immune Sera,
pubmed-meshheading:10225969-Mice,
pubmed-meshheading:10225969-Molecular Sequence Data,
pubmed-meshheading:10225969-Streptococcus pyogenes
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pubmed:year |
1999
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pubmed:articleTitle |
New protective antigen of group A streptococci.
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pubmed:affiliation |
Veterans Affairs Medical Center, Memphis, Tennessee 38104, USA. james.dale@med.va.gov
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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