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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-5-18
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pubmed:abstractText |
Pretreatment of human peripheral blood monocytes with a very low concentration (0.1 ng/ml) of Porphyromonas gingivalis lipopolysaccharides (LPS) resulted in a significant decrease of interleukin-6 (IL-6) production, but not IL-8 production, by restimulation of a high concentration (1 microg/ml) of the same LPS. In contrast, the same pretreatment with Escherichia coli LPS resulted in the enhanced production of both IL-6 and IL-8 after restimulation. The selective induction by P. gingivalis LPS tolerance of IL-6 production developed in a time-dependent manner during the primary culture. P. gingivalis LPS-pretreated cells were also refractory to a high-dose E. coli LPS restimulation in terms of IL-6 production. The expression of IL-6 mRNA decreased 10 h after restimulation of P. gingivalis LPS-pretreated monocytes. Furthermore, an up-regulation of anti-inflammatory cytokine IL-10 upon a second high-dose LPS rechallenge occurred at the same time point in the pretreated cells. We studied the role of IL-10 in the process of IL-6 down-regulation. Neutralization by an anti-IL-10 polyclonal antibody prevented IL-6 down-regulation in P. gingivalis LPS-pretreated monocytes, whereas IL-8 production was not affected. Addition of exogenous IL-10 during the high-dose LPS stimulation of untreated cells substituted for the LPS pretreatment and resulted in the inhibition of IL-6 production in a dose-dependent manner. A higher dose of IL-10 was required to suppress IL-8 synthesis from monocytes. Our data suggest that IL-10 mediates IL-6 down-regulation in P. gingivalis LPS-tolerant monocytes in an autocrine manner.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-1429677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-1551689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-1831827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-1940369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-2541142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-2663993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-2789513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-3280611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-3500852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-3555304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-6222173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-7515924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-7583350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-7583353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-7688664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-7692988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-7722463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-8025687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-8112323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-8119492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-8318675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-8386517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-8405442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-8426119,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10225868-9558074
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2153-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10225868-Base Sequence,
pubmed-meshheading:10225868-Cytokines,
pubmed-meshheading:10225868-DNA Primers,
pubmed-meshheading:10225868-Humans,
pubmed-meshheading:10225868-Inflammation Mediators,
pubmed-meshheading:10225868-Interleukin-10,
pubmed-meshheading:10225868-Interleukin-6,
pubmed-meshheading:10225868-Interleukin-8,
pubmed-meshheading:10225868-Lipopolysaccharides,
pubmed-meshheading:10225868-Monocytes,
pubmed-meshheading:10225868-Neutralization Tests,
pubmed-meshheading:10225868-Porphyromonas gingivalis,
pubmed-meshheading:10225868-RNA, Messenger
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pubmed:year |
1999
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pubmed:articleTitle |
Autoregulatory effect of interleukin-10 on proinflammatory cytokine production by Porphyromonas gingivalis lipopolysaccharide-tolerant human monocytes.
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pubmed:affiliation |
Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. simauti@dent.osaka-u.ac.jp
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pubmed:publicationType |
Journal Article,
In Vitro
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