Linked Life Data

10223199

Source:http://linkedlifedata.com/resource/pubmed/id/10223199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-5-20
pubmed:abstractText
The importance of epidermal growth factor (EGF) in both normal and malignant mammary gland development are presented in these studies. Initial findings demonstrated that in the absence of ovarian hormones, EGF had a significant proliferative effect on mammary epithelial cells. To determine whether mammary epithelial cells grown with EGF, in the absence of ovarian hormones, could be transformed by N-methyl-N-nitrosourea (MNU), female ovariectomized Lewis rats were implanted with pellets containing EGF for 1 week and then treated with MNU for initiation. Two days after MNU treatment, ovaries were implanted and EGF pellets were removed from all ovariectomized groups in order to promote carcinogenesis. The mammary carcinoma incidence of the EGF-stimulated group (90%) was not significantly different from the intact group (100%). The mammary cancer morphology of EGF-treated carcinomas was either ductal carcinoma or cribriform adenocarcinoma, whereas intact animals developed mainly papillary and occasional cribriform carcinomas. Fifty-eight percent of the carcinomas from the EGF group were ovarian hormone-independent compared with 10% of carcinomas from the intact group. These results demonstrate that EGF-induced proliferation during initiation with MNU was sufficient to induce the transformation of mammary carcinomas in the absence of ovarian hormones. The hormonal dependency of these EGF-induced carcinomas were different compared with MNU-initiated mammary carcinomas in intact rats.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
677-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10223199-Adenocarcinoma, pubmed-meshheading:10223199-Animals, pubmed-meshheading:10223199-Carcinoma, Ductal, Breast, pubmed-meshheading:10223199-Carcinoma, Papillary, pubmed-meshheading:10223199-Cell Division, pubmed-meshheading:10223199-DNA Mutational Analysis, pubmed-meshheading:10223199-Epidermal Growth Factor, pubmed-meshheading:10223199-Estradiol, pubmed-meshheading:10223199-Estrogens, pubmed-meshheading:10223199-Female, pubmed-meshheading:10223199-Genes, ras, pubmed-meshheading:10223199-Mammary Neoplasms, Experimental, pubmed-meshheading:10223199-Methylnitrosourea, pubmed-meshheading:10223199-Neoplasm Proteins, pubmed-meshheading:10223199-Neoplasms, Hormone-Dependent, pubmed-meshheading:10223199-Ovariectomy, pubmed-meshheading:10223199-Ovary, pubmed-meshheading:10223199-Polymerase Chain Reaction, pubmed-meshheading:10223199-Progesterone, pubmed-meshheading:10223199-Rats, pubmed-meshheading:10223199-Rats, Inbred Lew, pubmed-meshheading:10223199-Rats, Sprague-Dawley, pubmed-meshheading:10223199-Receptors, Estrogen, pubmed-meshheading:10223199-Receptors, Progesterone
pubmed:year
1999
pubmed:articleTitle
Induction of mammary carcinomas by N-methyl-N-nitrosourea in ovariectomized rats treated with epidermal growth factor.
pubmed:affiliation
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA. nandilab@lsa.berkeley.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.