| pubmed-article:10222211 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0085490 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0085236 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0014264 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0064841 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C1333196 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C1523116 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:10222211 | lifeskim:mentions | umls-concept:C0702240 | lld:lifeskim |
| pubmed-article:10222211 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10222211 | pubmed:dateCreated | 1999-8-9 | lld:pubmed |
| pubmed-article:10222211 | pubmed:abstractText | In bovine alveolar macrophages (BAMs), exposure to leukotoxin (Lkt) and endotoxin (LPS) from Pasteurella haemolytica results in expression of inflammatory cytokine genes and intracellular calcium ([Ca2+]i) elevation. Leukotoxin from P. haemolytica interacts only with leukocytes and platelets from ruminant species. Upregulation of cytokine genes in different cells by LPS involves activation of the transcription factor NF-kappaB (NF-kappaB), resulting in its translocation from the cytoplasm to the nucleus. Using immunocytochemical staining and confocal imaging, we studied whether NF-kappaB activation represents a common mechanism for the expression of multiple cytokine genes in BAMs (Lkt-susceptible cells) stimulated with Lkt and LPS. Bovine pulmonary artery endothelial cells and porcine alveolar macrophages were used as nonsusceptible cells. The role of Ca2+ and tyrosine kinases in NF-kappaB activation and inflammatory cytokine gene expression was studied, since an inhibitor of tyrosine kinases attenuates LPS-induced [Ca2+]i elevation in BAMs. The results are summarized as follows: (a) Lkt induced NF-kappaB activation and [Ca2+]i elevation only in BAMs, while LPS effects were demonstrable in all cell types; (b) chelation of [Ca2+]i blocked NF-kappaB activation and IL-1beta, TNFalpha, and IL-8 mRNA expression; and (c) tyrosine kinase inhibitor herbimycin A blocked expression of all three cytokine genes in BAMs stimulated with Lkt, while only the expression of IL-1beta was blocked in BAMs stimulated with LPS. We conclude that cytokine gene expression in BAMs requires NF-kappaB activation and [Ca2+]i elevation, and Lkt effects exhibit cell type- and species specificity. | lld:pubmed |
| pubmed-article:10222211 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10222211 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10222211 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10222211 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10222211 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10222211 | pubmed:month | May | lld:pubmed |
| pubmed-article:10222211 | pubmed:issn | 0882-4010 | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:SieckG CGC | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:MaheswaranS... | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:JeyaseelanSS | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:KannanM SMS | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:AbrahamsenM... | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:PrakashY SYS | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:HsuanS LSL | lld:pubmed |
| pubmed-article:10222211 | pubmed:author | pubmed-author:MalazdrewichC... | lld:pubmed |
| pubmed-article:10222211 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10222211 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10222211 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:10222211 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10222211 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10222211 | pubmed:pagination | 263-73 | lld:pubmed |
| pubmed-article:10222211 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10222211 | pubmed:meshHeading | pubmed-meshheading:10222211... | lld:pubmed |
| pubmed-article:10222211 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10222211 | pubmed:articleTitle | Pasteurella haemolytica leukotoxin and endotoxin induced cytokine gene expression in bovine alveolar macrophages requires NF-kappaB activation and calcium elevation. | lld:pubmed |
| pubmed-article:10222211 | pubmed:affiliation | Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA. | lld:pubmed |
| pubmed-article:10222211 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10222211 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:10222211 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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