Source:http://linkedlifedata.com/resource/pubmed/id/10222211
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
1999-8-9
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| pubmed:abstractText |
In bovine alveolar macrophages (BAMs), exposure to leukotoxin (Lkt) and endotoxin (LPS) from Pasteurella haemolytica results in expression of inflammatory cytokine genes and intracellular calcium ([Ca2+]i) elevation. Leukotoxin from P. haemolytica interacts only with leukocytes and platelets from ruminant species. Upregulation of cytokine genes in different cells by LPS involves activation of the transcription factor NF-kappaB (NF-kappaB), resulting in its translocation from the cytoplasm to the nucleus. Using immunocytochemical staining and confocal imaging, we studied whether NF-kappaB activation represents a common mechanism for the expression of multiple cytokine genes in BAMs (Lkt-susceptible cells) stimulated with Lkt and LPS. Bovine pulmonary artery endothelial cells and porcine alveolar macrophages were used as nonsusceptible cells. The role of Ca2+ and tyrosine kinases in NF-kappaB activation and inflammatory cytokine gene expression was studied, since an inhibitor of tyrosine kinases attenuates LPS-induced [Ca2+]i elevation in BAMs. The results are summarized as follows: (a) Lkt induced NF-kappaB activation and [Ca2+]i elevation only in BAMs, while LPS effects were demonstrable in all cell types; (b) chelation of [Ca2+]i blocked NF-kappaB activation and IL-1beta, TNFalpha, and IL-8 mRNA expression; and (c) tyrosine kinase inhibitor herbimycin A blocked expression of all three cytokine genes in BAMs stimulated with Lkt, while only the expression of IL-1beta was blocked in BAMs stimulated with LPS. We conclude that cytokine gene expression in BAMs requires NF-kappaB activation and [Ca2+]i elevation, and Lkt effects exhibit cell type- and species specificity.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Cytotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Exotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/leukotoxin
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| pubmed:status |
MEDLINE
|
| pubmed:month |
May
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| pubmed:issn |
0882-4010
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
| pubmed:issnType |
Print
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| pubmed:volume |
26
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
263-73
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:10222211-Animals,
pubmed-meshheading:10222211-Bacterial Toxins,
pubmed-meshheading:10222211-Calcium,
pubmed-meshheading:10222211-Cattle,
pubmed-meshheading:10222211-Cells, Cultured,
pubmed-meshheading:10222211-Cytokines,
pubmed-meshheading:10222211-Cytotoxins,
pubmed-meshheading:10222211-Endothelium, Vascular,
pubmed-meshheading:10222211-Exotoxins,
pubmed-meshheading:10222211-Gene Expression Regulation,
pubmed-meshheading:10222211-Interleukin-1,
pubmed-meshheading:10222211-Interleukin-8,
pubmed-meshheading:10222211-Lipopolysaccharides,
pubmed-meshheading:10222211-Macrophages, Alveolar,
pubmed-meshheading:10222211-Mannheimia haemolytica,
pubmed-meshheading:10222211-NF-kappa B,
pubmed-meshheading:10222211-Protein-Tyrosine Kinases,
pubmed-meshheading:10222211-Swine,
pubmed-meshheading:10222211-Tumor Necrosis Factor-alpha
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| pubmed:year |
1999
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| pubmed:articleTitle |
Pasteurella haemolytica leukotoxin and endotoxin induced cytokine gene expression in bovine alveolar macrophages requires NF-kappaB activation and calcium elevation.
|
| pubmed:affiliation |
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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