Linked Life Data

10217599

Source:http://linkedlifedata.com/resource/pubmed/id/10217599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15-16
pubmed:dateCreated
1999-7-8
pubmed:abstractText
We analyzed the humoral immune response elicited by hepatitis C virus (HCV) E2 protein expressed in vivo after injection of plasmid DNA into mice and rhesus macaques. Three plasmids were used for immunization: a plasmid containing the entire sequence of the E2 and p7 genes (pE2); a plasmid encoding a truncated form of the E2 protein targeted to the cell surface (pE2surf); a control plasmid (pDisplay) lacking an HCV insert. Each plasmid was injected intramuscularly into 5 mice and intraepidermally (via gene gun) into 5 mice. Immunization was repeated three times at three week intervals. Five macaques were injected intramuscularly (two with pE2, two with pE2surf and one with pDisplay) and immunization was repeated after 8 weeks. All mice immunized via gene gun with pE2 or pE2surf developed anti-E2. The animals immunized with pE2surf developed an earlier and stronger humoral immune response than those immunized with pE2. Only 2 of the mice injected by the intramuscular route, both immunized with pE2surf, developed detectable anti-E2. One of the two macaques immunized with pE2 and both macaques immunized with pE2surf developed anti-E2; the humoral immune response was much stronger in the animals immunized with pE2surf. Our results suggest that presentation of HCV E2 on the cell surface may increase its immunogenicity while preserving its ability to react with antibodies generated during a natural infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0264-410X
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1992-2002
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10217599-Animals, pubmed-meshheading:10217599-Antibodies, Viral, pubmed-meshheading:10217599-Biolistics, pubmed-meshheading:10217599-Blotting, Western, pubmed-meshheading:10217599-Cell Line, pubmed-meshheading:10217599-Cell Membrane, pubmed-meshheading:10217599-Female, pubmed-meshheading:10217599-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:10217599-Hepacivirus, pubmed-meshheading:10217599-Immunoglobulin G, pubmed-meshheading:10217599-Injections, Intramuscular, pubmed-meshheading:10217599-Macaca, pubmed-meshheading:10217599-Male, pubmed-meshheading:10217599-Mice, pubmed-meshheading:10217599-Mice, Inbred BALB C, pubmed-meshheading:10217599-Plasmids, pubmed-meshheading:10217599-Recombinant Proteins, pubmed-meshheading:10217599-Transfection, pubmed-meshheading:10217599-Vaccines, DNA, pubmed-meshheading:10217599-Viral Envelope Proteins, pubmed-meshheading:10217599-Viral Vaccines
pubmed:year
1999
pubmed:articleTitle
DNA immunization of mice and macaques with plasmids encoding hepatitis C virus envelope E2 protein expressed intracellularly and on the cell surface.
pubmed:affiliation
Hepatitis Viruses and Molecular Hepatitis Sections, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0740, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't