Source:http://linkedlifedata.com/resource/pubmed/id/10214952
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
1999-5-5
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pubmed:abstractText |
Reverse transcriptase of human immunodeficiency virus type I is a vitalenzyme in the HIV-1 replication cycle and an attractive target of attempts to arrest a primary viral infection. We designed a vector for eukaryotic expression of the 66 kDa subunit of reverse transcriptase under the control of the immediate early cytomegalovirus promoter. Efficient transient expression of the 66 kDa subunit of reverse transcriptase was achieved in a variety of cells. Immunostaining of the transfected cells revealed the cytoplasmatic localization of reverse transcriptase. Reverse transcriptase activity was detected in all transfected cell lines. Injection of this plasmid encoding the 66 kDa subunit of reverse transcriptase into mice resulted in strong reverse transcriptase-specific immune responses indicating that the 66 kDa subunit of reverse transcriptase is expressed in vivo. Sera from DNA-immunized mice inhibited reverse transcription in vitro.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
447
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
232-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10214952-3T3 Cells,
pubmed-meshheading:10214952-AIDS Vaccines,
pubmed-meshheading:10214952-Animals,
pubmed-meshheading:10214952-COS Cells,
pubmed-meshheading:10214952-Cell Line,
pubmed-meshheading:10214952-Cercopithecus aethiops,
pubmed-meshheading:10214952-Cytomegalovirus,
pubmed-meshheading:10214952-DNA, Recombinant,
pubmed-meshheading:10214952-Gene Expression,
pubmed-meshheading:10214952-Genetic Vectors,
pubmed-meshheading:10214952-HIV Antibodies,
pubmed-meshheading:10214952-HIV Reverse Transcriptase,
pubmed-meshheading:10214952-HIV-1,
pubmed-meshheading:10214952-Humans,
pubmed-meshheading:10214952-Immunization,
pubmed-meshheading:10214952-Mice,
pubmed-meshheading:10214952-Mice, Inbred C57BL,
pubmed-meshheading:10214952-Molecular Weight,
pubmed-meshheading:10214952-Protein Conformation,
pubmed-meshheading:10214952-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Eukaryotic expression of enzymatically active human immunodeficiency virus type 1 reverse transcriptase.
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pubmed:affiliation |
D.I. Ivanovsky Institute of Virology, Moscow, Russia. maria.isaguliants@solna.mail.telia.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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