Source:http://linkedlifedata.com/resource/pubmed/id/10213391
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
1999-7-9
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| pubmed:abstractText |
Spermatogonial-Sertoli cell co-cultures, prepared from sexually immature rats (7-10 days old) and maintained for experimental purposes for a maximum period of time of eight days, were used to determine whether Sertoli cell geometry can influence spermatogonial cell growth, viability and differentiation. We have found that when Sertoli cells are allowed to stretch, spermatogonial cell cohorts attached to Sertoli cell surfaces remain viable and exhibit typical cell oscillatory movements with a maximal oscillation radial length of 0.8 microm throughout the duration of the experiments. However, spermatogonial cell viability decreased when Sertoli cells were compelled to contract by preventing cell spreading onto a non-adhesive substrate. A video-microscopy analysis of spermatogonial cells progenies cocultured with contracted Sertoli cells revealed that conjoined members of the cohorts displayed a typical apoptotic sequence preceded by vigorous oscillatory cell movements (maximal oscillation radial length: 1.5 microm) followed by the release of apoptotic bodies and cessation of cell movements. This sequence of events occurred in a single cell. Upon completion of this sequence, another member of the cohort initiated the same cell death course until all members completed the cell death sequence. A similar apoptotic sequence was observed following addition of Fas (CD95/APO-1) antibody (ligand agonist) to the cocultures. Fragmentation of the actin-containing cytoskeleton was observed by indirect immunofluorescence in apoptotic spermatogonial cell cohorts, independent from the activating mechanism. We conclude that by forcing Sertoli cells to contract or by adding an apoptosis inducer to the cocultures, individual members of a spermatogonial cell cohort switch on a death (apoptosis) program in a coordinated fashion.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
1058-8388
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
214
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
361-71
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10213391-Actins,
pubmed-meshheading:10213391-Animals,
pubmed-meshheading:10213391-Antigens, CD95,
pubmed-meshheading:10213391-Apoptosis,
pubmed-meshheading:10213391-Cell Death,
pubmed-meshheading:10213391-Cells, Cultured,
pubmed-meshheading:10213391-Coculture Techniques,
pubmed-meshheading:10213391-Cytoskeleton,
pubmed-meshheading:10213391-Fluorescent Antibody Technique,
pubmed-meshheading:10213391-Male,
pubmed-meshheading:10213391-Microscopy, Video,
pubmed-meshheading:10213391-Rats,
pubmed-meshheading:10213391-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:10213391-Sertoli Cells,
pubmed-meshheading:10213391-Spermatogonia,
pubmed-meshheading:10213391-Stem Cells,
pubmed-meshheading:10213391-Time Factors
|
| pubmed:year |
1999
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| pubmed:articleTitle |
Cell death patterns of the rat spermatogonial cell progeny induced by sertoli cell geometric changes and Fas (CD95) agonist.
|
| pubmed:affiliation |
Department of Cell Biology and Anatomical Sciences, The City University of New York Medical School, New York 10031, USA.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|