Linked Life Data

10210692

Source:http://linkedlifedata.com/resource/pubmed/id/10210692

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-7-28
pubmed:abstractText
Hamsters breathed, nose-only, for 13 weeks, 5 days/week, 6 hr/day, either man-made vitreous fiber (MMVF)10a, MMVF33, or long amosite asbestos at approximately 300 World Health Organization (WHO) fibers/cc or long amosite at 25 WHO fibers/cc. [World Health Organization fibers are longer than 5 microm and thicker than 3 microm, with aspect ratio >3.] After sacrifice, fiber burden was estimated (left lungs) by ashing and scanning electron microscopy (ashing/SEM) or (right middle lobes) by confocal laser scanning microscopy (CLSM) in situ. In situ CLSM also provided three-dimensional views of fibers retained, undisturbed, in lung tissue. Fibers of each type were lodged in alveoli and small airways, especially at airway bifurcations, and were seen fully or partly engulfed by alveolar macrophages. Amosite fibers penetrated into and through alveolar septa. Length densities of fibers in parenchyma (total length of fiber per unit volume of lung) were estimated stereologically from fiber transsections counted on two-dimensional optical sections and were 30.5, 25.3, 20.0, and 81.6 mm/mm3 for MMVF10a, MMVF33, and low- and high-dose amosite, respectively. Lengths of individual fibers were measured in three dimensions by tracking individual fibers through series of optical sections. Length distributions of amosite fibers aerosolized, but before inhalation versus after retention in the lung were similar, whether determined by ashing/SEM or in situ CLSM. In contrast, the fraction of short MMVF10a and MMVF33 fibers increased and the geometric mean fiber lengths of both MMVFs decreased by approximately 60% during retention. Most likely due to fiber deposition pattern and differences in sampling, fiber burdens [MMVF10a, MMVF33, and amosite (high dose; 269 WHO fibers/cc)] determined by ashing/SEM were 1.4, 1. 5, and 3.5 times greater, respectively, than those calculated from in situ CLSM data. In situ CLSM is able to provide detailed information about the anatomic sites of fiber retention and also fiber lengths and burdens in good agreement with ashing/SEM results.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-1851478, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-2872911, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-372672, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-4364384, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-6267971, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-6625350, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-6636189, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-7181276, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-7389688, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-7882930, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-8390950, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-8666112, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-9400728, http://linkedlifedata.com/resource/pubmed/commentcorrection/10210692-9707503
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0091-6765
pubmed:author
pubmed:issnType
Print
pubmed:volume
107
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
367-75
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
In situ microscopic analysis of asbestos and synthetic vitreous fibers retained in hamster lungs following inhalation.
pubmed:affiliation
Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't