Linked Life Data

10209337

Source:http://linkedlifedata.com/resource/pubmed/id/10209337

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-5-27
pubmed:abstractText
Fluorescence in situ hybridisation (FISH) technique with chromosome specific library (CSL) DNA probes for all human chromosomes were used to study about 9000 micronuclei (MN) in normal and idoxuridine (IUdR)-treated lymphocyte cultures of female and male donors. In addition, MN rates and structural chromosome aberrations were scored in Giemsa-stained chromosome spreads of these cultures. IUdR treatment (40 microg/ml) induced on the average a 12-fold increase of the MN rate. Metaphase analysis revealed no distinct increase of chromosome breaks but a preferential decondensation at chromosome 9q12 (28-79%) and to a lower extend at 1q12 (8-21%). Application of FISH technique with CSL probes to one male and one female untreated proband showed that all human chromosomes except chromosome 12 (and to a striking high frequency chromosomes 9, X and Y) occurred in spontaneous MN. In cultures containing IUdR, the chromosomal spectrum found in MN was reduced to 10 chromosomes in the male and 13 in the female proband. Eight chromosomes (2, 6, 12, 13, 14, 15, 17 and 18) did not occur in MN of both probands. On the contrary chromosomes 1 and especially 9 were found much more frequently in the MN of IUdR-treated cultures than in MN of control cultures. DAPI-staining revealed heterochromatin signals in most of the IUdR-induced MN. In an additional study, spontaneous and IUdR-induced MN were investigated in lymphocytes of another female donor using CSL probes only for chromosomes 1, 6, 9, 15, 16 and X. The results confirmed the previous finding that chromosomes 1 and 9 occur very often in MN after IUdR-treatment. The results indicate that decondensation of heterochromatic regions on chromosomes 1 and 9 caused by IUdR treatment strongly correlates with MN formation by these chromosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-5107
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
440
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
147-56
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10209337-Azure Stains, pubmed-meshheading:10209337-Cell Culture Techniques, pubmed-meshheading:10209337-Chromosome Aberrations, pubmed-meshheading:10209337-Chromosome Breakage, pubmed-meshheading:10209337-Chromosomes, Human, Pair 1, pubmed-meshheading:10209337-Chromosomes, Human, Pair 9, pubmed-meshheading:10209337-DNA Probes, pubmed-meshheading:10209337-Female, pubmed-meshheading:10209337-Gene Library, pubmed-meshheading:10209337-Heterochromatin, pubmed-meshheading:10209337-Humans, pubmed-meshheading:10209337-Idoxuridine, pubmed-meshheading:10209337-In Situ Hybridization, Fluorescence, pubmed-meshheading:10209337-Indoles, pubmed-meshheading:10209337-Lymphocytes, pubmed-meshheading:10209337-Male, pubmed-meshheading:10209337-Metaphase, pubmed-meshheading:10209337-Micronuclei, Chromosome-Defective, pubmed-meshheading:10209337-Nucleic Acid Synthesis Inhibitors, pubmed-meshheading:10209337-Sex Factors
pubmed:year
1999
pubmed:articleTitle
Comparison of spontaneous and idoxuridine-induced micronuclei by chromosome painting.
pubmed:affiliation
Abt. Humanbiologie und Humangenetik der Universität, Postfach 3049, D-67653, Kaiserslautern, Germany. efauth@rhrk.uni-kl.de
pubmed:publicationType
Journal Article, Comparative Study