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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-6-29
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pubmed:abstractText |
The precursor of non-amyloid beta protein component of Alzheimer's disease amyloid (NACP/alpha-synuclein), found in Lewy bodies of Parkinson's disease (PD), is a presynaptic protein genetically linked to some familial types PD. Mechanisms of abnormal NACP/alpha-synuclein aggregation in neurodegenerative diseases are unclear. Since oxidative stress might play a role in PD pathogenesis, we investigated the role of iron and peroxide in NACP/alpha-synuclein aggregation. Immunoblot analysis showed that human NACP/alpha-synuclein (but not beta-synuclein) aggregated in the presence of ferric ion and was inhibited by the iron chelator deferoxamine. Ferrous ion was not effective by itself, but it potentially aggregated NACP/alpha-synuclein in the presence of hydrogen peroxide. NACP/ alpha-synuclein aggregates displayed strong thioflavine-S and congo-red reactivity, reminiscent of amyloid. This study suggests that NACP/alpha-synuclein aggregation might be closely related to oxidative reactions which may play a critical role in neurodegeneration in disorders with Lewy bodies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Ferric Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Ferrous Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SNCA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SNCB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Synucleins,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Synuclein,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Synuclein,
http://linkedlifedata.com/resource/pubmed/chemical/ferric chloride,
http://linkedlifedata.com/resource/pubmed/chemical/ferrous chloride
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0959-4965
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
717-21
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10208537-Alzheimer Disease,
pubmed-meshheading:10208537-Amyloid,
pubmed-meshheading:10208537-Catalysis,
pubmed-meshheading:10208537-Chlorides,
pubmed-meshheading:10208537-Ferric Compounds,
pubmed-meshheading:10208537-Ferrous Compounds,
pubmed-meshheading:10208537-Humans,
pubmed-meshheading:10208537-Nerve Tissue Proteins,
pubmed-meshheading:10208537-Oxidation-Reduction,
pubmed-meshheading:10208537-Oxidative Stress,
pubmed-meshheading:10208537-Recombinant Proteins,
pubmed-meshheading:10208537-Synucleins,
pubmed-meshheading:10208537-alpha-Synuclein,
pubmed-meshheading:10208537-beta-Synuclein
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pubmed:year |
1999
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pubmed:articleTitle |
Oxidative stress induces amyloid-like aggregate formation of NACP/alpha-synuclein in vitro.
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pubmed:affiliation |
Department of Neurosciences, University of California San Diego, La Jolla 92093-0624, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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