| pubmed-article:10206659 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0018557 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0029045 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0207230 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0385934 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:10206659 | lifeskim:mentions | umls-concept:C1334043 | lld:lifeskim |
| pubmed-article:10206659 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10206659 | pubmed:dateCreated | 1999-4-13 | lld:pubmed |
| pubmed-article:10206659 | pubmed:abstractText | Injections of cytosolic preparations from mammalian sperm into oocytes have been shown to trigger calcium [Ca2+]i oscillations and initiate activation of development. Recently, a protein isolated from hamster sperm has been suggested to be involved in the generation of these oscillations and it was named "oscillin." The human homologue of hamster oscillin is glucosamine 6-phosphate isomerase (GPI, EC no. 5.3.1.10), an enzyme so far described to be involved in hexose phosphate metabolism. To assess the role of GPI on Ca2+ signaling, a human recombinant protein was generated in a prokaryotic system and injected into fura-2-dextran-loaded metaphase II (MII) mouse oocytes. Injection of recombinant GPI failed to induce Ca2+ responses in 12/12 injected MII oocytes despite the fact that the recombinant GPI was active as assessed by an enzymatic assay. Injection of buffer (0/6 oocytes) or fructose-6-phosphate, a product of GPI enzymatic reaction (0/5 oocytes), also failed to initiate Ca2+ responses. Conversely, injections of sperm cytosolic factor induced [Ca2+]i oscillations in all 17/17 oocytes. In addition, injection of recombinant GPI or GPI mRNA failed to induce parthenogenetic activation (0/30 oocytes). Immunofluorescence studies using an anti-GPI polyclonal antibody (GK) resulted in localization of GPI to the sperm's equatorial region. Incubation of the GK antibody with sperm extracts failed to block the [Ca2+]i responses induced by these extracts. Moreover, near complete depletion of GPI from sperm fractions by immunoprecipitation did not impair the ability of these fractions to induce [Ca2+]i oscillations. In summary, our results support the role of a sperm cytosolic component(s) in the generation of [Ca2+]i oscillations during mammalian fertilization, although a protein other than GPI/oscillin is likely to be the active calcium releasing factor. | lld:pubmed |
| pubmed-article:10206659 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10206659 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10206659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10206659 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10206659 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10206659 | pubmed:issn | 1040-452X | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:RosenwaksZZ | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:WuHH | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:FissoreR ARA | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:ReinM RMR | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:PalermoG DGD | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:ColomberoL... | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:WolnyY MYM | lld:pubmed |
| pubmed-article:10206659 | pubmed:author | pubmed-author:ErgünBB | lld:pubmed |
| pubmed-article:10206659 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10206659 | pubmed:volume | 52 | lld:pubmed |
| pubmed-article:10206659 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10206659 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10206659 | pubmed:pagination | 277-87 | lld:pubmed |
| pubmed-article:10206659 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:10206659 | pubmed:meshHeading | pubmed-meshheading:10206659... | lld:pubmed |
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| pubmed-article:10206659 | pubmed:meshHeading | pubmed-meshheading:10206659... | lld:pubmed |
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| pubmed-article:10206659 | pubmed:meshHeading | pubmed-meshheading:10206659... | lld:pubmed |
| pubmed-article:10206659 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10206659 | pubmed:articleTitle | Human glucosamine-6-phosphate isomerase, a homologue of hamster oscillin, does not appear to be involved in Ca2+ release in mammalian oocytes. | lld:pubmed |
| pubmed-article:10206659 | pubmed:affiliation | The Center for Reproductive Medicine and Infertility, New York Hospital-Cornell Medical Center, New York 10021, USA. | lld:pubmed |
| pubmed-article:10206659 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10206659 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
