Source:http://linkedlifedata.com/resource/pubmed/id/10205890
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
1999-5-6
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| pubmed:abstractText |
The effects of four mycotoxins, T-2 toxin, deoxynivalenol (DON), ochratoxin A (OTA) and fumonisin B1 (FB1) on the response of bovine peripheral blood mononuclear cells (PBM) in vitro to the mitogens concanavalin A (Con A), phytohaemagglutinin A (PHA) and pokeweed mitogen (PWM) were assayed after 4 days' incubation using 3H-thymidine uptake and the MTT bioassay. The concentrations of mycotoxin required to reduce the proliferative response of PBM by 50% for Con A, PHA and PWM as measured by 3H-thymidine incorporation was for T-2 toxin 0.30, 0.40 and 0.18 ng ml-1; for DON 0.07, 0.09 and 0.04 microgram ml-1; for OTA 0.10, 0.20 and 0.15 microgram ml-1, and for FB1 35, 18 and 11 micrograms ml-1 respectively. The concentrations of mycotoxin required to reduce the proliferative response of PBM by 50% for Con A, PHA and PWM as measured by the MTT bioassay were for T-2 toxin 2.0, 2.0 and 1.0 mg ml-1; for DON 0.70, 0.50 and 0.50 microgram ml-1; OTA 1.5, 1.5 and 1.5 micrograms ml-1; and FB1 > 50, > 50 and 20 micrograms ml-1 respectively. Further cytotoxicity assays including the LDH bioassay and Trypan blue exclusion were performed only on Con A-stimulated PBM cells after 72 h incubation. With the LDH-bioassay the 50% inhibition levels were T-2 toxin 0.3 ng ml-1, DON 0.4 microgram ml-1, OTA 1.4 micrograms ml-1 and FB1 3.5 micrograms ml-1; for Trypan blue uptake the 50% inhibition levels were T-2 toxin 5 ng ml-1, DON 2.3 micrograms ml-1 and OTA 4 micrograms ml-1 respectively.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carboxylic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Fumonisins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Mycotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Ochratoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/Pokeweed Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/T-2 Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Trichothecenes,
http://linkedlifedata.com/resource/pubmed/chemical/deoxynivalenol,
http://linkedlifedata.com/resource/pubmed/chemical/fumonisin B1,
http://linkedlifedata.com/resource/pubmed/chemical/ochratoxin A
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0301-486X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
143
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
105-11
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10205890-Animals,
pubmed-meshheading:10205890-Carboxylic Acids,
pubmed-meshheading:10205890-Cattle,
pubmed-meshheading:10205890-Cell Division,
pubmed-meshheading:10205890-Concanavalin A,
pubmed-meshheading:10205890-Female,
pubmed-meshheading:10205890-Fumonisins,
pubmed-meshheading:10205890-Immunity, Cellular,
pubmed-meshheading:10205890-Leukocytes, Mononuclear,
pubmed-meshheading:10205890-Mitogens,
pubmed-meshheading:10205890-Mycotoxins,
pubmed-meshheading:10205890-Ochratoxins,
pubmed-meshheading:10205890-Phytohemagglutinins,
pubmed-meshheading:10205890-Pokeweed Mitogens,
pubmed-meshheading:10205890-T-2 Toxin,
pubmed-meshheading:10205890-Time Factors,
pubmed-meshheading:10205890-Trichothecenes
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| pubmed:year |
1998
|
| pubmed:articleTitle |
The effects of four mycotoxins on the mitogen stimulated proliferation of bovine peripheral blood mononuclear cells in vitro.
|
| pubmed:affiliation |
Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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