Linked Life Data

10205880

Source:http://linkedlifedata.com/resource/pubmed/id/10205880

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-5-28
pubmed:abstractText
Lipid free-radical oxidation has been studied in vivo in the mitochondrial fractions of the liver of rats fed RoseOx (carnosic acid nutritional supplement) by measuring chemiluminescence. The kinetics of the lipid chemiluminescence in rats fed RoseOx are significantly different from those of the control. The intensity of the chemiluminescence fast flash decreases by 45% (p < 0.01), which indicates a reduction of lipid peroxides. The time between fast and slow flashes increases by 96% (p < 0.05), which indicates a higher content of antioxidants in the lipid membrane. The in vitro experiments in rat liver mitochondrial fraction display more effective antioxidant action of alpha-tocopherol in 1 microM concentration than 1 microM carnosic acid by an increase of the time between fast and slow chemiluminescence flashes (p < 0.01). However, the higher antioxidant activity of 1 microM carnosic acid by a decrease of intensity of the chemiluminescence fast (p < 0.05) and slow (p < 0.05) flashes in comparison with alpha-tocopherol is revealed in these experimental conditions in vitro. Carnosic acid has antioxidant effects on homogeneous oxidation in vitro as well. The chemiluminescence of methyl oleate initiated by 2,2'-azobis(2-methylpropionitrile) decreases by 25% (p < 0.01) in the presence of 13.5 microM carnosic acid. 13.5 microM alpha-tocopherol decreases the methyl oleate chemiluminescence by 45%. A higher antioxidant activity of alpha-tocopherol in comparison with carnosic acid (p < 0.001) is found in this system. These results indicate that RoseOx reduces free-radical-induced lipid peroxidation in vivo. In vitro data show that carnosic acid has direct action as an antioxidant, rather than as a membrane-structure modifier.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1011-1344
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-7
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Chemiluminescence determination of the in vivo and in vitro antioxidant activity of RoseOx and carnosic acid.
pubmed:affiliation
Coenzyme Biochemistry Department, A.V. Palladin Institute of Biochemistry, Ukrainian National Academy of Sciences, Kiev, Ukraine. akuzm@mail.kar.net
pubmed:publicationType
Journal Article