|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
1999-6-9
|
|
pubmed:abstractText |
1. The receptors which mediate the effects of calcitonin gene-related peptide (CGRP), amylin and adrenomedullin on the guinea-pig vas deferens have been investigated. 2. All three peptides cause concentration dependant inhibitions of the electrically stimulated twitch response (pD2s for CGRP, amylin and adrenomedullin of 7.90+/-0.11, 7.70+/-0.19 and 7.25+/-0.10 respectively). 3. CGRP8-37 (1 microM) and AC187 (10 microM) showed little antagonist activity against adrenomedullin. 4. Adrenomedullin22-52 by itself inhibited the electrically stimulated contractions of the vas deferens and also antagonized the responses to CGRP, amylin and adrenomedullin. 5. [125I]-adrenomedullin labelled a single population of binding sites in vas deferens membranes with a pIC50 of 8.91 and a capacity of 643 fmol mg(-1). Its selectivity profile was adrenomedullin> AC187>CGRP=amylin. It was clearly distinct from a site labelled by [125I]-CGRP (pIC50=8.73, capacity=114 fmol mg(-1), selectivity CGRP>amylin=AC187>adrenomedullin). [125I]-amylin bound to two sites with a total capacity of 882 fmol mg(-1). 6. Although CGRP has been shown to act at a CGRP2 receptor on the vas deferens with low sensitivity to CGRP8-37, this antagonist displaced [125I]-CGRP with high affinity from vas deferens membranes. This affinity was unaltered by increasing the temperature from 4 degrees C to 25 degrees C, suggesting the anomalous behaviour of CGRP8-37 is not due to temperature differences between binding and functional assays.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-1310282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-1313730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-1322107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-2164085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-2553933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-7643091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-7688224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-7713143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-7720662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-7813564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-7957618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8387282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8404688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8548167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8549809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8578616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8626685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8761478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8828574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8882637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-8957226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-9039014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-9165042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-9195480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-9388596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-9396787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10205019-9620797
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenomedullin,
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin Gene-Related Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Islet Amyloid Polypeptide,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenomedullin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Calcitonin Gene-Related...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/calcitonin gene-related peptide...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0007-1188
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
126
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1276-82
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10205019-Adrenomedullin,
pubmed-meshheading:10205019-Amyloid,
pubmed-meshheading:10205019-Animals,
pubmed-meshheading:10205019-Binding, Competitive,
pubmed-meshheading:10205019-Calcitonin Gene-Related Peptide,
pubmed-meshheading:10205019-Guinea Pigs,
pubmed-meshheading:10205019-Iodine Radioisotopes,
pubmed-meshheading:10205019-Islet Amyloid Polypeptide,
pubmed-meshheading:10205019-Male,
pubmed-meshheading:10205019-Membrane Proteins,
pubmed-meshheading:10205019-Muscle Contraction,
pubmed-meshheading:10205019-Peptide Fragments,
pubmed-meshheading:10205019-Peptides,
pubmed-meshheading:10205019-Radioligand Assay,
pubmed-meshheading:10205019-Receptors, Adrenomedullin,
pubmed-meshheading:10205019-Receptors, Calcitonin Gene-Related Peptide,
pubmed-meshheading:10205019-Receptors, Peptide,
pubmed-meshheading:10205019-Vas Deferens,
pubmed-meshheading:10205019-Vasodilator Agents
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Characterization of receptors for calcitonin gene-related peptide and adrenomedullin on the guinea-pig vas deferens.
|
|
pubmed:affiliation |
Pharmaceutical Sciences Institute, Aston University, Birmingham, England. poynerdr@aston.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
