| pubmed-article:10204689 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C0013081 | lld:lifeskim |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C0022702 | lld:lifeskim |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C0599896 | lld:lifeskim |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:10204689 | lifeskim:mentions | umls-concept:C1947906 | lld:lifeskim |
| pubmed-article:10204689 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10204689 | pubmed:dateCreated | 1999-6-3 | lld:pubmed |
| pubmed-article:10204689 | pubmed:abstractText | Agonist-activated beta2-adrenoceptors rapidly internalize and then recycle to the cell surface, however chronic agonist eventually causes receptor downregulation. To characterize beta2-adrenoceptor trafficking kinetics and intracellular sorting during downregulation, human embryonic kidney cells expressing epitope-tagged receptors were examined by radioligand binding with (+/-)-[3H]4-(3-tertiarybutylamino-2-hydroxypropoxy)-benzimidazole- 2-on hydrochloride ([3H]CGP12177) and immunofluorescence microscopy. The first-order receptor recycling rate constant declined after 18 h of agonist compared with 15 min (0.05 min(-1) vs. 0.12 min(-1)), thus increasing the intracellular transit time (20.0 min vs. 8.3 min). There was also a reduction in the rate of receptor endocytosis and a decline in the total number of receptors. Although the intracellular receptor fraction did not increase between 15 min and 18 h of agonist, some receptors moved irreversibly into a protease-containing compartment while retaining radioligand binding activity. Our results indicate that beta2-adrenoceptor downregulation is associated principally with an increased intracellular transit time during recycling. This could promote the diversion of receptors into protease-containing compartments, where there is an irreversible commitment to downregulation prior to loss of radioligand binding activity. | lld:pubmed |
| pubmed-article:10204689 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10204689 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10204689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10204689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10204689 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10204689 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10204689 | pubmed:issn | 0014-2999 | lld:pubmed |
| pubmed-article:10204689 | pubmed:author | pubmed-author:MooreR HRH | lld:pubmed |
| pubmed-article:10204689 | pubmed:author | pubmed-author:KnollB JBJ | lld:pubmed |
| pubmed-article:10204689 | pubmed:author | pubmed-author:DayGG | lld:pubmed |
| pubmed-article:10204689 | pubmed:author | pubmed-author:RosenfeldJ... | lld:pubmed |
| pubmed-article:10204689 | pubmed:author | pubmed-author:HallH SHS | lld:pubmed |
| pubmed-article:10204689 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10204689 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:10204689 | pubmed:volume | 369 | lld:pubmed |
| pubmed-article:10204689 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10204689 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10204689 | pubmed:pagination | 113-23 | lld:pubmed |
| pubmed-article:10204689 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:10204689 | pubmed:meshHeading | pubmed-meshheading:10204689... | lld:pubmed |
| pubmed-article:10204689 | pubmed:meshHeading | pubmed-meshheading:10204689... | lld:pubmed |
| pubmed-article:10204689 | pubmed:meshHeading | pubmed-meshheading:10204689... | lld:pubmed |
| pubmed-article:10204689 | pubmed:meshHeading | pubmed-meshheading:10204689... | lld:pubmed |
| pubmed-article:10204689 | pubmed:meshHeading | pubmed-meshheading:10204689... | lld:pubmed |
| pubmed-article:10204689 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10204689 | pubmed:articleTitle | Specific changes in beta2-adrenoceptor trafficking kinetics and intracellular sorting during downregulation. | lld:pubmed |
| pubmed-article:10204689 | pubmed:affiliation | Department of Pediatrics (Pulmonary), Baylor College of Medicine, Houston, TX 77030, USA. | lld:pubmed |
| pubmed-article:10204689 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10204689 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10204689 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10204689 | lld:pubmed |
