Linked Life Data

10204579

Source:http://linkedlifedata.com/resource/pubmed/id/10204579

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-4-29
pubmed:abstractText
The fluorescent probes dichlorofluorescin (DCFH), dihydrorhodamine (DHR), and hydroethidine (HE) allow convenient assay of alveolar macrophage (AM) oxidant responses to enviromental particulates and pathogens. We sought to more precisely define the relationship of these measures of oxidant stress to production of pro-inflammatory cytokines. Normal AMs were challenged in vitro with a panel of soluble or particulate stimuli in the presence of DCFH, HE, or DHR. Flow cytometry measured cell-associated fluorescence and relative particle uptake. Tumor necrosis factor alpha and macrophage inflammatory protein 2 expression were quantitated in the same experiments. We observed variable and complex correlations between intracellular oxidant production as reported by these probes and subsequent cytokine response, including examples of striking discordance (e.g., lipopolysaccharide induced large cytokine responses with minimal probe oxidation, whereas fly ash particles caused marked oxidation of DCFH but trivial TNF release; TiO2 caused oxidation of DHR and HE, but not DCFH, and also did not increase cytokine production). Although fluorescent probes offer many advantages in analysis of intracellular oxidant responses, the data indicate that they cannot be used reliably as quantitative predictors of AM cytokine responses to environmental particulates or other stimuli.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0741-5400
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
499-507
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10204579-Animals, pubmed-meshheading:10204579-Blotting, Northern, pubmed-meshheading:10204579-Cells, Cultured, pubmed-meshheading:10204579-Chemokine CXCL2, pubmed-meshheading:10204579-Cricetinae, pubmed-meshheading:10204579-Cytokines, pubmed-meshheading:10204579-Female, pubmed-meshheading:10204579-Fluoresceins, pubmed-meshheading:10204579-Fluorescent Dyes, pubmed-meshheading:10204579-Intracellular Fluid, pubmed-meshheading:10204579-Lung, pubmed-meshheading:10204579-Macrophage Activation, pubmed-meshheading:10204579-Macrophages, Alveolar, pubmed-meshheading:10204579-Mesocricetus, pubmed-meshheading:10204579-Mitochondria, pubmed-meshheading:10204579-Monokines, pubmed-meshheading:10204579-Oxidants, pubmed-meshheading:10204579-Phenanthridines, pubmed-meshheading:10204579-RNA, Messenger, pubmed-meshheading:10204579-Rats, pubmed-meshheading:10204579-Respiratory Burst, pubmed-meshheading:10204579-Tumor Necrosis Factor-alpha
pubmed:year
1999
pubmed:articleTitle
Intracellular oxidant production and cytokine responses in lung macrophages: evaluation of fluorescent probes.
pubmed:affiliation
Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115, USA. aimrich@hsph.harvard.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.