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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-4-30
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pubmed:abstractText |
Fast synaptic transmission depends on the selective ionic permeability of transmitter-gated ion channels. Here we show changes in the ion selectivity of neuronal P2X transmitter-gated cation channels as a function of time (on the order of seconds) and previous ATP exposure. Heterologously expressed P2X2, P2X2/P2X3 and P2X4 channels as well as native neuronal P2X channels possess various combinations of mono- or biphasic responses and permeability changes, measured by NMDG+ and fluorescent dye. Furthermore, in P2X4 receptors, this ability to alter ion selectivity can be increased or decreased by altering an amino-acid residue thought to line the ion permeation pathway, identifying a region that governs this activity-dependent change.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Benzoxazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Cations,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Meglumine,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Quinolinium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic P2X4,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/YO-PRO 1
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1097-6256
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
322-30
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10204538-Adenosine Triphosphate,
pubmed-meshheading:10204538-Amino Acid Substitution,
pubmed-meshheading:10204538-Animals,
pubmed-meshheading:10204538-Benzoxazoles,
pubmed-meshheading:10204538-Cations,
pubmed-meshheading:10204538-Cell Membrane Permeability,
pubmed-meshheading:10204538-Cells, Cultured,
pubmed-meshheading:10204538-DNA, Complementary,
pubmed-meshheading:10204538-Fluorescent Dyes,
pubmed-meshheading:10204538-Ion Transport,
pubmed-meshheading:10204538-Meglumine,
pubmed-meshheading:10204538-Mutagenesis, Site-Directed,
pubmed-meshheading:10204538-Nerve Tissue Proteins,
pubmed-meshheading:10204538-Patch-Clamp Techniques,
pubmed-meshheading:10204538-Quinolinium Compounds,
pubmed-meshheading:10204538-Rats,
pubmed-meshheading:10204538-Receptors, Nicotinic,
pubmed-meshheading:10204538-Receptors, Purinergic P2,
pubmed-meshheading:10204538-Receptors, Purinergic P2X2,
pubmed-meshheading:10204538-Receptors, Purinergic P2X3,
pubmed-meshheading:10204538-Receptors, Purinergic P2X4,
pubmed-meshheading:10204538-Recombinant Fusion Proteins,
pubmed-meshheading:10204538-Time Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Neuronal P2X transmitter-gated cation channels change their ion selectivity in seconds.
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pubmed:affiliation |
Division of Biology, California Institute of Technology, Pasadena 91125, USA. balkhakh@its.caltech.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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