rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
3
|
pubmed:dateCreated |
1999-5-27
|
pubmed:abstractText |
A cDNA clone encoding 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH) was isolated from a human fetal liver cDNA library. It contained the open reading frame of 2,709 base pairs and predicted a protein comprising 902 amino acids with a calculated molecular weight of 98,700 Da. The deduced protein showed about 93.6% homology (90.5% identity, 3.1% favored substitutions) when compared with rat 10-FTHFDH. The distribution of 10-FTHFDH transcript in various human tissues was studied by Northern blot analysis using poly(A+) RNAs from different tissues. The 10-FTHFDH transcript with an approximate size of 2.7 kb was mainly expressed in human kidney, skeletal muscle, and liver and rarely expressed in other tissues.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
1039-9712
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
47
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
407-15
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:10204077-Amino Acid Sequence,
pubmed-meshheading:10204077-Base Sequence,
pubmed-meshheading:10204077-Cloning, Molecular,
pubmed-meshheading:10204077-DNA, Complementary,
pubmed-meshheading:10204077-Humans,
pubmed-meshheading:10204077-Liver,
pubmed-meshheading:10204077-Molecular Sequence Data,
pubmed-meshheading:10204077-Oxidoreductases Acting on CH-NH Group Donors,
pubmed-meshheading:10204077-RNA, Messenger,
pubmed-meshheading:10204077-Restriction Mapping,
pubmed-meshheading:10204077-Sequence Homology, Amino Acid
|
pubmed:year |
1999
|
pubmed:articleTitle |
Isolation and characterization of cDNA clone for human liver 10-formyltetrahydrofolate dehydrogenase.
|
pubmed:affiliation |
Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|