Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-5-7
pubmed:databankReference
pubmed:abstractText
A clone expressing an immunoreactive protein with an apparent molecular mass of 44 kDa was selected from an Ehrlichia chaffeensis Arkansas genomic library by probing with anti-E. chaffeensis hyperimmune mouse ascitic fluid. Nucleotide sequencing revealed an open reading frame (ORF) capable of encoding a 198-amino-acid polypeptide. The ORF contained four imperfect, direct, tandem 90-bp repeats. The nucleotide and deduced amino acid sequences did not show close homologies to entries in the molecular databases. PCR with primers whose sequences matched the sequences flanking the ORF was performed with DNA samples extracted from cell cultures infected with nine different isolates of E. chaffeensis, blood samples from seven patients with monocytic ehrlichiosis, and Amblyomma americanum ticks collected in four different states. The resulting amplicons varied in length, containing three to six repeat units. This gene, designated the variable-length PCR target, is useful for PCR detection of E. chaffeensis and differentiation of isolates.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-1374076, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-1471733, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-1581187, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-1757543, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-1757557, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-2809255, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-3029590, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-681182, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-7616529, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-7665632, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8004184, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8101510, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8132352, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8317710, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8357086, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8807206, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8903194, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-8906920, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9031621, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9086141, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9126818, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9230387, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9230803, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9242329, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9316896, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9423849, http://linkedlifedata.com/resource/pubmed/commentcorrection/10203503-9647746
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1447-53
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Molecular cloning and characterization of the Ehrlichia chaffeensis variable-length PCR target: an antigen-expressing gene that exhibits interstrain variation.
pubmed:affiliation
Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jws3@cdc.gov
pubmed:publicationType
Journal Article