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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1999-5-6
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pubmed:abstractText |
Cytokine regulation of endocytic activity in primary human macrophages was studied to define ultrastructural changes and mechanisms of pinocytic regulation associated with cytokines secreted by activated T cells. The effects of IFN-gamma (type 1) and IL-4/IL-13 and IL-10 (type 2) cytokines on fluid phase and mannose receptor-mediated endocytosis were assessed by horseradish peroxidase and colloidal gold-BSA uptake and computer-assisted morphometric analysis. IL-4 and IL-13 enhanced fluid phase pinocytosis and mannose receptor-mediated uptake by activation of phosphatidylinositol 3-kinase. Inhibition of actin assembly showed that both cytokines exerted actin-dependent and -independent effects. Ultrastructurally, IL-4 and IL-13 increased tubular vesicle formation underneath the plasma membrane and at pericentriolar sites, concurrent with decreased particle sorting to lysosomes. By contrast, IL-10 or IFN-gamma decreased both fluid phase pinocytosis and mannose receptor-mediated uptake. IFN-gamma stimulated increased particle sorting to perinuclear lysosomes, while IL-10 decreased this activity. In summary, our data document differential effects on macrophage endocytic functions by type 1 or type 2 cytokines associated with induction and effector pathways in immunity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Gold Colloid,
http://linkedlifedata.com/resource/pubmed/chemical/Horseradish Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose-Binding Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/mannose receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4606-13
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10202000-Actins,
pubmed-meshheading:10202000-Cell Nucleus,
pubmed-meshheading:10202000-Endocytosis,
pubmed-meshheading:10202000-Endosomes,
pubmed-meshheading:10202000-Gold Colloid,
pubmed-meshheading:10202000-Golgi Apparatus,
pubmed-meshheading:10202000-Horseradish Peroxidase,
pubmed-meshheading:10202000-Humans,
pubmed-meshheading:10202000-Interferon-gamma,
pubmed-meshheading:10202000-Interleukin-10,
pubmed-meshheading:10202000-Interleukin-13,
pubmed-meshheading:10202000-Interleukin-4,
pubmed-meshheading:10202000-Lectins, C-Type,
pubmed-meshheading:10202000-Lysosomes,
pubmed-meshheading:10202000-Macrophage Activation,
pubmed-meshheading:10202000-Macrophages,
pubmed-meshheading:10202000-Mannose-Binding Lectins,
pubmed-meshheading:10202000-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:10202000-Receptors, Cell Surface,
pubmed-meshheading:10202000-Th1 Cells,
pubmed-meshheading:10202000-Th2 Cells
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pubmed:year |
1999
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pubmed:articleTitle |
Type 1 and type 2 cytokine regulation of macrophage endocytosis: differential activation by IL-4/IL-13 as opposed to IFN-gamma or IL-10.
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pubmed:affiliation |
The Wistar Institute, Philadelphia, PA 19104, USA. montaner@wistar.upenn.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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