Linked Life Data

10200526

Source:http://linkedlifedata.com/resource/pubmed/id/10200526

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1999-5-27
pubmed:abstractText
DNA fragmentation is a hallmark of apoptosis, and has been viewed as a short-lived process (<hour) that immediately precedes cell elimination. However, use of sensitive techniques like in situ end-labelling plus (ISEL+) has indicated that the period between the initiation of detectable fragmentation and cell elimination could be longer (days). To address this possibility, we used a model system of cell death and replacement, the murine small intestinal villus. Pulses of 5-bromo-2'-deoxyuridine were used to follow quantitatively cohorts of cells from their generation in the crypts to their elimination at the villus tips, resulting in a temporal 'yard-stick' where position on the villus indicated time before cell elimination; these data allowed a mathematical description of cell movement and clearance. Combining these data with ISEL+ quantitation, enterocytes were found to commence and maintain DNA fragmentation 2 - 3 days before elimination, a phenomenon that likely has relevance to studies on apoptosis also in other systems.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1350-9047
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
702-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Onset of apoptotic DNA fragmentation can precede cell elimination by days in the small intestinal villus.
pubmed:affiliation
Department of Pharmacology, School of Medicine, University of California, San Diego, California, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't