Source:http://linkedlifedata.com/resource/pubmed/id/10199788
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1999-4-21
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pubmed:abstractText |
Prophet of Pit-1 (Prop-1), which is a paired-like homeodomain transcription factor, is capable of binding to sites in an early enhancer of the Pit-1 gene and regulating its expression. According to a previous report, Prop-1 messenger RNA (mRNA) is expressed in the developing pituitary gland before Pit-1 mRNA expression and maximum expression are observed at e 12.0. After e 14.5, Prop-1 mRNA expression rapidly decreases, and only trace amounts of mRNA are detectable in adult mouse pituitary. Human Pit-1 is expressed considerably, not only in normal adult pituitary but also in pituitary adenomas, so we studied human Prop-1 gene expression in adult pituitary and pituitary adenomas. We also cloned human Prop-1 complementary DNA (cDNA) and sequenced the Prop-1 cDNAs in pituitary adenomas. The amino acid sequence of human Prop-1 cDNA that we cloned was identical to that of the previously reported sequence, except Thr substituted at codon 142 instead of Ala. This amino acid substitution is considered to be a polymorphism because it did not alter transcriptional activity, and 7 of 28 alleles were Ala. Human Prop-1 transcript was detected in normal adult pituitary, by Northern blot analysis, and in all pituitary adenomas examined by RT-PCR analysis. The expression of human Prop-1 in pituitary adenomas was confirmed by in situ hybridization in one of the somatotroph adenomas. The sequence analysis of human Prop-1 cDNAs in these pituitary adenomas revealed that there were no mutations, except 5 silent nucleic acid substitutions, suggesting that mutations of Prop-1 gene do not represent a frequent mechanism of human pituitary tumorigenesis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-972X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1414-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10199788-Adenoma,
pubmed-meshheading:10199788-Adult,
pubmed-meshheading:10199788-Base Sequence,
pubmed-meshheading:10199788-Cloning, Molecular,
pubmed-meshheading:10199788-Homeodomain Proteins,
pubmed-meshheading:10199788-Humans,
pubmed-meshheading:10199788-Molecular Sequence Data,
pubmed-meshheading:10199788-Mutation,
pubmed-meshheading:10199788-Pituitary Gland,
pubmed-meshheading:10199788-Pituitary Neoplasms,
pubmed-meshheading:10199788-RNA, Messenger,
pubmed-meshheading:10199788-Transcription, Genetic
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pubmed:year |
1999
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pubmed:articleTitle |
Characterization of Prophet of Pit-1 gene expression in normal pituitary and pituitary adenomas in humans.
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pubmed:affiliation |
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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