Source:http://linkedlifedata.com/resource/pubmed/id/10199581
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
1999-4-20
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| pubmed:abstractText |
Interleukin (IL) 12 is a heterodimeric cytokine which stimulates IFN-gamma production and promotes the development of type 1 T helper cells and T cytotoxic cells from their respective precursors. We have described previously that contact allergens such as 2,4-dinitrochlorobenzene (DNCB), and respiratory allergens such as trimellitic anhydride (TMA) induce discrete type 1 and type 2 cytokine secretion patterns, respectively, following repeated topical exposure of BALB/c strain mice. Under such conditions, we have now examined production by draining LNC of the inducible subunit of IL-12 (p40) and p40 and p35 subunit mRNA expression. Cultured LNC prepared from mice treated with DNCB secreted significantly more IL-12 p40 protein than did TMA- or vehicle-activated LNC, such differences becoming more pronounced as the duration of culture increased. Maximal levels of mRNA expression of the IL-12 p40 subunit were observed after 6-24 h of culture in all treatment groups and declined thereafter. Somewhat higher levels were induced following exposure to DNCB, these differences only reached statistical significance at 24 h of culture. Expression of this subunit by LNC from all treatment groups declined with time in culture. Levels of IL-12 p35 mRNA expression were comparable in cultured LNC prepared from allergen and vehicle treated mice and remained constant throughout the entire culture period. These data indicate that the divergent T cell cytokine responses seen in response to contact and respiratory allergens are associated with differential production of IL-12 p40 protein in the absence of substantial changes in the expression of mRNA for either subunit.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetone,
http://linkedlifedata.com/resource/pubmed/chemical/Allergens,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrochlorobenzene,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/NCF4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phthalic Anhydrides,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Oils,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/olive oil,
http://linkedlifedata.com/resource/pubmed/chemical/trimellitic anhydride
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
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| pubmed:issn |
0300-483X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
132
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
57-66
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10199581-Acetone,
pubmed-meshheading:10199581-Administration, Topical,
pubmed-meshheading:10199581-Allergens,
pubmed-meshheading:10199581-Animals,
pubmed-meshheading:10199581-Cells, Cultured,
pubmed-meshheading:10199581-DNA Primers,
pubmed-meshheading:10199581-DNA-Binding Proteins,
pubmed-meshheading:10199581-Dinitrochlorobenzene,
pubmed-meshheading:10199581-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10199581-Female,
pubmed-meshheading:10199581-Interleukin-12,
pubmed-meshheading:10199581-Lymph Nodes,
pubmed-meshheading:10199581-Mice,
pubmed-meshheading:10199581-Mice, Inbred BALB C,
pubmed-meshheading:10199581-NADPH Oxidase,
pubmed-meshheading:10199581-Phthalic Anhydrides,
pubmed-meshheading:10199581-Plant Oils,
pubmed-meshheading:10199581-Proteins,
pubmed-meshheading:10199581-RNA, Messenger,
pubmed-meshheading:10199581-Reverse Transcriptase Polymerase Chain Reaction
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| pubmed:year |
1999
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| pubmed:articleTitle |
Analysis of interleukin 12 protein production and mRNA expression in mice exposed topically to chemical allergens.
|
| pubmed:affiliation |
Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, UK. vicky.warbrick@ctl.zeneca.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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