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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0017428,
umls-concept:C0017725,
umls-concept:C0204727,
umls-concept:C0205147,
umls-concept:C0205409,
umls-concept:C0597484,
umls-concept:C0598850,
umls-concept:C1123019,
umls-concept:C1420200,
umls-concept:C1880022,
umls-concept:C2699249
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pubmed:issue |
2
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pubmed:dateCreated |
1999-5-24
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pubmed:databankReference |
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pubmed:abstractText |
d-Glucose and non-metabolisable analogues of D-glucose regulate the expression of intestinal SGLT1 at both transcriptional and post-transcriptional levels. In order to investigate the molecular mechanisms involved in the transcriptional regulation of the ovine intestinal SGLT1 gene, we have isolated an upstream element of about 1 kb in size. This DNA fragment contains a TATA box motif, 48 bp upstream of the transcriptional start site and includes transcription factor binding sites for HNF-1 and AP-2. We have shown that the ovine SGLT1 promoter fragment can drive the transcription of a reporter gene when transfected into the epithelial cell lines STC-1 and LLC-PK1, which endogenously express SGLT1. Deletion analyses of the promoter indicate that -66/+21 bp proximal sequence directs the highest level of reporter gene activity. There are one and possibly two sites of transcriptional suppression.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HNF1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/HNF1B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-beta,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SLC5A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Glucose Transporter 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-2,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
533-7
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:10198246-5' Untranslated Regions,
pubmed-meshheading:10198246-Animals,
pubmed-meshheading:10198246-Base Sequence,
pubmed-meshheading:10198246-Cell Line,
pubmed-meshheading:10198246-Cloning, Molecular,
pubmed-meshheading:10198246-DNA-Binding Proteins,
pubmed-meshheading:10198246-Epithelial Cells,
pubmed-meshheading:10198246-Gene Expression Regulation,
pubmed-meshheading:10198246-Genes, Reporter,
pubmed-meshheading:10198246-Goats,
pubmed-meshheading:10198246-Hepatocyte Nuclear Factor 1,
pubmed-meshheading:10198246-Hepatocyte Nuclear Factor 1-alpha,
pubmed-meshheading:10198246-Hepatocyte Nuclear Factor 1-beta,
pubmed-meshheading:10198246-Humans,
pubmed-meshheading:10198246-Intestines,
pubmed-meshheading:10198246-Membrane Glycoproteins,
pubmed-meshheading:10198246-Molecular Sequence Data,
pubmed-meshheading:10198246-Monosaccharide Transport Proteins,
pubmed-meshheading:10198246-Nuclear Proteins,
pubmed-meshheading:10198246-Promoter Regions, Genetic,
pubmed-meshheading:10198246-RNA, Messenger,
pubmed-meshheading:10198246-Repressor Proteins,
pubmed-meshheading:10198246-Response Elements,
pubmed-meshheading:10198246-Sequence Deletion,
pubmed-meshheading:10198246-Sequence Homology, Nucleic Acid,
pubmed-meshheading:10198246-Sodium-Glucose Transporter 1,
pubmed-meshheading:10198246-TATA Box,
pubmed-meshheading:10198246-Transcription Factor AP-2,
pubmed-meshheading:10198246-Transcription Factors,
pubmed-meshheading:10198246-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Isolation and characterization of a genomic region upstream from the ovine Na+/d-glucose cotransporter (SGLT1) cDNA.
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pubmed:affiliation |
Department of Veterinary Preclinical Sciences, University of Liverpool, Brownlow Hill, L69 3BX, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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