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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-5-24
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pubmed:abstractText |
Proteasome 26S must recognize the PEST region-containing C-terminus of mammalian ornithine decarboxylase (ODC) monomer to proceed with degradation. We have detected PEST regions in both termini of mammalian histidine decarboxylase (HDC). In the present report, a chimaeric ODC/HDC was used to elucidate whether the PEST region-containing C-termini of ODC and HDC are exchangeable. Wild-type rat ODC had an expected antizyme and ATP-dependent degradation. This was not the case for both the chimaera and a C-terminus truncated rat ODC. Results suggest that the PEST region-containing C-terminus of rat HDC should have another role different to confering polypeptide availability to the proteasome.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
269-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10198201-Adenosine Triphosphate,
pubmed-meshheading:10198201-Amino Acid Sequence,
pubmed-meshheading:10198201-Animals,
pubmed-meshheading:10198201-Enzyme Stability,
pubmed-meshheading:10198201-Half-Life,
pubmed-meshheading:10198201-Histidine Decarboxylase,
pubmed-meshheading:10198201-Kinetics,
pubmed-meshheading:10198201-Ornithine Decarboxylase,
pubmed-meshheading:10198201-Peptide Hydrolases,
pubmed-meshheading:10198201-Proteasome Endopeptidase Complex,
pubmed-meshheading:10198201-Protein Biosynthesis,
pubmed-meshheading:10198201-Protein Conformation,
pubmed-meshheading:10198201-Rats,
pubmed-meshheading:10198201-Recombinant Fusion Proteins,
pubmed-meshheading:10198201-Sequence Deletion,
pubmed-meshheading:10198201-Structure-Activity Relationship,
pubmed-meshheading:10198201-Transcription, Genetic
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pubmed:year |
1999
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pubmed:articleTitle |
The pest regions containing C-termini of mammalian ornithine decarboxylase and histidine decarboxylase play different roles in protein degradation.
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pubmed:affiliation |
Facultad de Ciencias, Universidad de Málaga, Málaga, E-29071, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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