10198047

pubmed:Citation

4

We noted previously that certain aminoglycoside antibiotics inhibit the binding of coatomer to Golgi membranes in vitro. The inhibition is mediated in part by two primary amino groups present at the 1 and 3 positions of the 2-deoxystreptamine moiety of the antibiotics. These two amines appear to mimic the epsilon-amino groups present in the two lysine residues of the KKXX motif that is known to bind coatomer. Here we report the effects of 1, 3-cyclohexanebis(methylamine) (CBM) on secretion in vivo, a compound chosen for study because it contains primary amino groups that resemble those in 2-deoxystreptamine and it should penetrate lipid bilayers more readily than antibiotics. CBM inhibited coatomer binding to Golgi membranes in vitro and in vivo and inhibited secretion by intact cells. Despite depressed binding of coatomer in vivo, the Golgi complex retained its characteristic perinuclear location in the presence of CBM and did not fuse with the endoplasmic reticulum (ER). Transport from the ER to the Golgi was also not blocked by CBM. These data suggest that a full complement of coat protein I (COPI) on membranes is not critical for maintenance of Golgi integrity or for traffic from the ER to the Golgi but is necessary for transport through the Golgi to the plasma membrane.

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http://linkedlifedata.com/resource/pubmed/journal/9201390

http://linkedlifedata.com/resource/pubmed/chemical/2-deoxystreptamine, http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Protein Complex gamma..., http://linkedlifedata.com/resource/pubmed/chemical/Anti-Bacterial Agents, http://linkedlifedata.com/resource/pubmed/chemical/Coatomer Protein, http://linkedlifedata.com/resource/pubmed/chemical/Cyclohexanes, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hemagglutinin Glycoproteins..., http://linkedlifedata.com/resource/pubmed/chemical/Hexosamines, http://linkedlifedata.com/resource/pubmed/chemical/Mannosidases, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Methylamines, http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/mannosyl-oligosaccharide 1,3 -...

Apr

pubmed-author:DraperR KRK, pubmed-author:EydeR HRH, pubmed-author:HudsonR TRT, pubmed-author:IIYY, pubmed-author:KaoC YCY

10

Y

2009-11-18