Source:http://linkedlifedata.com/resource/pubmed/id/10197798
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
1999-7-15
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| pubmed:abstractText |
Graft-versus-host disease (GVHD), due to the presence of recipient-reactive T cells, limits the usefulness of bone marrow transplantation (BMT) and is a major contributor to patient mortality. To prevent GVHD, murine and human T cells were activated by antigen or mitogens and treated with a genetically engineered form of Pseudomonas exotoxin A (PE) directed against the IL-2 receptor. Treatment with the chimeric toxin eliminated alloreactive cytotoxic T lymphocytes (CTL) as determined by cytotoxicity and mixed lymphocyte culture assays. Precursor frequencies of alloreactive cytotoxic T cells and proliferative T cells were reduced up to 100-fold as shown by limiting dilution assays. Flow cytometric analyses revealed that treatment with the chimeric toxin completely eliminated CD25+ cells from the cultures. Toxin treatment had no significant effect on hematopoietic stem and progenitor cells as determined in vitro by colony-forming assays and in vivo by long-term hematopoietic recovery after 950 rad irradiation. Toxin treatment decreased GVHD in transplanted mice to less than 10% (as compared to 88% in untreated controls). Thus, it is possible to prevent life-threatening GVHD after BMT by using a CD25 receptor-directed toxin to eliminate host-reactive T cells from bone marrow grafts.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP Ribose Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Exotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors,
http://linkedlifedata.com/resource/pubmed/chemical/toxA protein, Pseudomonas aeruginosa
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0268-3369
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
137-44
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10197798-ADP Ribose Transferases,
pubmed-meshheading:10197798-Animals,
pubmed-meshheading:10197798-Bacterial Toxins,
pubmed-meshheading:10197798-Bone Marrow Transplantation,
pubmed-meshheading:10197798-Cell Count,
pubmed-meshheading:10197798-Exotoxins,
pubmed-meshheading:10197798-Female,
pubmed-meshheading:10197798-Graft vs Host Disease,
pubmed-meshheading:10197798-Humans,
pubmed-meshheading:10197798-Isoantibodies,
pubmed-meshheading:10197798-Mice,
pubmed-meshheading:10197798-Phenotype,
pubmed-meshheading:10197798-Pregnancy,
pubmed-meshheading:10197798-Pseudomonas aeruginosa,
pubmed-meshheading:10197798-Receptors, Interleukin-2,
pubmed-meshheading:10197798-Recombinant Proteins,
pubmed-meshheading:10197798-Stem Cells,
pubmed-meshheading:10197798-T-Lymphocytes,
pubmed-meshheading:10197798-Tissue Donors,
pubmed-meshheading:10197798-Virulence Factors
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| pubmed:year |
1999
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| pubmed:articleTitle |
Prevention of graft-versus-host disease (GVHD) by elimination of recipient-reactive donor T cells with recombinant toxins that target the interleukin 2 (IL-2) receptor.
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| pubmed:affiliation |
Department Microbiology and Immunology, University of Arizona, Tucson 85721, USA.
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| pubmed:publicationType |
Journal Article
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